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1h2o

From Proteopedia

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[[Image:1h2o.png|left|200px]]
 
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==SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W==
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The line below this paragraph, containing "STRUCTURE_1h2o", creates the "Structure Box" on the page.
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<StructureSection load='1h2o' size='340' side='right'caption='[[1h2o]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1h2o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prunus_avium Prunus avium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H2O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h2o OCA], [https://pdbe.org/1h2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h2o RCSB], [https://www.ebi.ac.uk/pdbsum/1h2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h2o ProSAT]</span></td></tr>
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{{STRUCTURE_1h2o| PDB=1h2o | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRU1_PRUAV PRU1_PRUAV]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h2/1h2o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h2o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific IgE cross-reacting with homologous food allergens. The tertiary structure of Pru av 1, the major cherry (Prunus avium) allergen, for example, is nearly identical with Bet v 1, the major birch (Betula verrucosa) pollen allergen. In order to define cross-reactive IgE epitopes, we generated and analysed mutants of Pru av 1 and Api g 1.0101, the major celery (Apium graveolens) allergen, by immunoblotting, EAST (enzyme allergosorbent test), CD and NMR spectroscopy. The mutation of Glu45 to Trp45 in the P-loop region, a known IgE epitope of Bet v 1, significantly reduced IgE binding to Pru av 1 in a subgroup of cherry-allergic patients. The backbone conformation of Pru av 1 wild-type is conserved in the three-dimensional structure of Pru av 1 Trp45, demonstrating that the side chain of Glu45 is involved in a cross-reactive IgE epitope. Accordingly, for a subgroup of celery-allergic patients, IgE binding to the homologous celery allergen Api g 1.0101 was enhanced by the mutation of Lys44 to Glu. The almost complete loss of IgE reactivity to the Pru av 1 Pro112 mutant is due to disruption of its tertiary structure. Neither the mutation Ala112 nor deletion of the C-terminal residues 155-159 influenced IgE binding to Pru av 1. In conclusion, the structure of the P-loop partially explains the cross-reactivity pattern, and modulation of IgE-binding by site-directed mutagenesis is a promising approach to develop hypo-allergenic variants for patient-tailored specific immunotherapy.
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===SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W===
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Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure.,Neudecker P, Lehmann K, Nerkamp J, Haase T, Wangorsch A, Fotisch K, Hoffmann S, Rosch P, Vieths S, Scheurer S Biochem J. 2003 Nov 15;376(Pt 1):97-107. PMID:12943529<ref>PMID:12943529</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12943529}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1h2o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12943529 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12943529}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1h2o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Prunus_avium Prunus avium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2O OCA].
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==Reference==
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<ref group="xtra">PMID:12943529</ref><ref group="xtra">PMID:11287426</ref><ref group="xtra">PMID:11824757</ref><ref group="xtra">PMID:11061231</ref><references group="xtra"/>
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[[Category: Prunus avium]]
[[Category: Prunus avium]]
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[[Category: Boehm, M.]]
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[[Category: Boehm M]]
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[[Category: Lehmann, K.]]
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[[Category: Lehmann K]]
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[[Category: Nerkamp, J.]]
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[[Category: Nerkamp J]]
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[[Category: Neudecker, P.]]
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[[Category: Neudecker P]]
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[[Category: Roesch, P.]]
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[[Category: Roesch P]]
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[[Category: Scheurer, S.]]
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[[Category: Scheurer S]]
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[[Category: Schweimer, K.]]
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[[Category: Schweimer K]]
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[[Category: Sticht, H.]]
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[[Category: Sticht H]]
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[[Category: Vieths, S.]]
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[[Category: Vieths S]]
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[[Category: Allergen]]
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[[Category: Heteronuclear nmr]]
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[[Category: Major cherry allergen]]
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[[Category: Pathogenesis-related protein]]
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[[Category: Plant defense]]
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[[Category: Structure]]
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Current revision

SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W

PDB ID 1h2o

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