1hk6

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[[Image:1hk6.jpg|left|200px]]
 
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==Ral binding domain from Sec5==
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The line below this paragraph, containing "STRUCTURE_1hk6", creates the "Structure Box" on the page.
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<StructureSection load='1hk6' size='340' side='right'caption='[[1hk6]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hk6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HK6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hk6 OCA], [https://pdbe.org/1hk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hk6 RCSB], [https://www.ebi.ac.uk/pdbsum/1hk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hk6 ProSAT]</span></td></tr>
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{{STRUCTURE_1hk6| PDB=1hk6 | SCENE= }}
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</table>
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== Function ==
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'''RAL BINDING DOMAIN FROM SEC5'''
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[https://www.uniprot.org/uniprot/EXOC2_MOUSE EXOC2_MOUSE] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.[UniProtKB:Q96KP1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hk6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hk6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The exocyst complex is involved in the final stages of exocytosis, when vesicles are targeted to the plasma membrane and dock. The regulation of exocytosis is vital for a number of processes, for example, cell polarity, embryogenesis, and neuronal growth formation. Regulation of the exocyst complex in mammals was recently shown to be dependent upon binding of the small G protein, Ral, to Sec5, a central component of the exocyst. This interaction is thought to be necessary for anchoring the exocyst to secretory vesicles. We have determined the structure of the Ral-binding domain of Sec5 and shown that it adopts a fold that has not been observed in a G protein effector before. This fold belongs to the immunoglobulin superfamily in a subclass known as IPT domains. We have mapped the Ral binding site on this domain and found that it overlaps with protein-protein interaction sites on other IPT domains but that it is completely different from the G protein-geranyl-geranyl interaction face of the Ig-like domain of the Rho guanine nucleotide dissociation inhibitor. This mapping, along with available site-directed mutagenesis data, allows us to predict how Ral and Sec5 may interact.
The exocyst complex is involved in the final stages of exocytosis, when vesicles are targeted to the plasma membrane and dock. The regulation of exocytosis is vital for a number of processes, for example, cell polarity, embryogenesis, and neuronal growth formation. Regulation of the exocyst complex in mammals was recently shown to be dependent upon binding of the small G protein, Ral, to Sec5, a central component of the exocyst. This interaction is thought to be necessary for anchoring the exocyst to secretory vesicles. We have determined the structure of the Ral-binding domain of Sec5 and shown that it adopts a fold that has not been observed in a G protein effector before. This fold belongs to the immunoglobulin superfamily in a subclass known as IPT domains. We have mapped the Ral binding site on this domain and found that it overlaps with protein-protein interaction sites on other IPT domains but that it is completely different from the G protein-geranyl-geranyl interaction face of the Ig-like domain of the Rho guanine nucleotide dissociation inhibitor. This mapping, along with available site-directed mutagenesis data, allows us to predict how Ral and Sec5 may interact.
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==About this Structure==
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Structure of the GTPase-binding domain of Sec5 and elucidation of its Ral binding site.,Mott HR, Nietlispach D, Hopkins LJ, Mirey G, Camonis JH, Owen D J Biol Chem. 2003 May 9;278(19):17053-9. Epub 2003 Mar 6. PMID:12624092<ref>PMID:12624092</ref>
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1HK6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HK6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the GTPase-binding domain of Sec5 and elucidation of its Ral binding site., Mott HR, Nietlispach D, Hopkins LJ, Mirey G, Camonis JH, Owen D, J Biol Chem. 2003 May 9;278(19):17053-9. Epub 2003 Mar 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12624092 12624092]
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</div>
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<div class="pdbe-citations 1hk6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Camonis JH]]
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[[Category: Camonis, J H.]]
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[[Category: Hopkins LJ]]
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[[Category: Hopkins, L J.]]
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[[Category: Mirey G]]
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[[Category: Mirey, G.]]
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[[Category: Mott HR]]
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[[Category: Mott, H R.]]
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[[Category: Nietlispach D]]
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[[Category: Nietlispach, D.]]
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[[Category: Owen D]]
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[[Category: Owen, D.]]
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[[Category: Immunoglobulin fold]]
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[[Category: Ipt ral sec5 exocyst,exocytosis,protein transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:56:07 2008''
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Current revision

Ral binding domain from Sec5

PDB ID 1hk6

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