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2bn8

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Solution Structure and interactions of the E .coli Cell Division Activator Protein CedA

Structural highlights

2bn8 is a 1 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CEDA_ECOLI Activates the cell division inhibited by chromosomal DNA over-replication.

Publication Abstract from PubMed

CedA is a protein that is postulated to be involved in the regulation of cell division in Escherichia coli and related organisms; however, little biological data about its possible mode of action are available. Here we present a three-dimensional structure of this protein as determined by NMR spectroscopy. The protein is made up of four antiparallel beta-strands, an alpha-helix, and a large unstructured stretch of residues at the N-terminus. It shows structural similarity to a family of DNA-binding proteins which interact with dsDNA via a three-stranded beta-sheet, suggesting that CedA may be a DNA-binding protein. The putative binding surface of CedA is predominantly positively charged with a number of basic residues surrounding a groove largely dominated by aromatic residues. NMR chemical shift perturbations and gel-shift experiments performed with CedA confirm that the protein binds dsDNA, and its interaction is mediated primarily via the beta-sheet.

Solution structure and interactions of the Escherichia coli cell division activator protein CedA.,Chen HA, Simpson P, Huyton T, Roper D, Matthews S Biochemistry. 2005 May 10;44(18):6738-44. PMID:15865419^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen HA, Simpson P, Huyton T, Roper D, Matthews S. Solution structure and interactions of the Escherichia coli cell division activator protein CedA. Biochemistry. 2005 May 10;44(18):6738-44. PMID:15865419 doi:http://dx.doi.org/10.1021/bi0500269

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bn8"

@@ Line 1: / Line 1: @@
 ==Solution Structure and interactions of the E .coli Cell Division Activator Protein CedA==
-<StructureSection load='2bn8' size='340' side='right' caption='[[2bn8]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='2bn8' size='340' side='right'caption='[[2bn8]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bn8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BN8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bn8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BN8 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn8 OCA], [http://pdbe.org/2bn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bn8 RCSB], [http://www.ebi.ac.uk/pdbsum/2bn8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bn8 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn8 OCA], [https://pdbe.org/2bn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bn8 RCSB], [https://www.ebi.ac.uk/pdbsum/2bn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bn8 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CEDA_ECOLI CEDA_ECOLI]] Activates the cell division inhibited by chromosomal DNA over-replication.
+[https://www.uniprot.org/uniprot/CEDA_ECOLI CEDA_ECOLI] Activates the cell division inhibited by chromosomal DNA over-replication.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Chen, H A]]
+[[Category: Escherichia coli]]
-[[Category: Huyton, T]]
+[[Category: Large Structures]]
-[[Category: Matthews, S]]
+[[Category: Chen HA]]
-[[Category: Roper, D]]
+[[Category: Huyton T]]
-[[Category: Simpson, P]]
+[[Category: Matthews S]]
-[[Category: Ceda]]
+[[Category: Roper D]]
-[[Category: Cell cycle protein]]
+[[Category: Simpson P]]
-[[Category: Cell division activator protein]]

2bn8

From Proteopedia

Current revision

Solution Structure and interactions of the E .coli Cell Division Activator Protein CedA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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