2iue

From Proteopedia

(Difference between revisions)

Current revision

Pactolus I-domain: Functional Switching of the Rossmann Fold

Structural highlights

2iue is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ITB2L_MOUSE During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Murine Pactolus is a neutrophil-specific single chain glycoprotein that plays a role as an apoptosis marker for macrophages. The extracellular region of the protein shows strong sequence similarities to integrin beta-subunits. Critical sequence modifications differentiate its function when compared to the integrin family. We show experimentally that Pactolus I-domain does not bind divalent metal ions, indicating that ligand binding is not mediated through a metal ion-dependent adhesion site (MIDAS). NMR data was used to map secondary structure and the strand pairing within the beta-sheet to confirm an overall Rossmann fold topology. Homology modeling enhanced by the NMR data was used to determine the overall structure, with two key loop insertions/deletions (insertion 2 and SDL) that distinguish the Pactolus I-domain from the integrin alpha I-domain and beta I-domains. NMR peak exchange broadening is observed due to dimerization, correlating to the beta I-domain and beta propeller heterodimerization region within the integrin headpiece. Two unique N-linked glycosylation sites (Asn151 and Asn230) within this region disrupt dimerization and may account for why Pactolus is not found to associate with an alpha-subunit. These changes in quaternary structure, ligand binding loops, glycosylation, and metal sites illustrate how evolution has rapidly and effectively altered key aspects of the integrin beta-subunit to derive a protein of novel function on an existing protein scaffold.

Pactolus I-domain: functional switching of the Rossmann fold.,Sen M, Legge GB Proteins. 2007 Aug 15;68(3):626-35. PMID:17523188^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hojgaard A, Close R, Dunn DM, Weiss RB, Weis JJ, Weis JH. Altered localization of CXCL13 expressing cells in mice deficient in Pactolus following an inflammatory stimulus. Immunology. 2006 Oct;119(2):212-23. Epub 2006 Jul 12. PMID:16836649 doi:http://dx.doi.org/IMM2426
↑ Sen M, Legge GB. Pactolus I-domain: functional switching of the Rossmann fold. Proteins. 2007 Aug 15;68(3):626-35. PMID:17523188 doi:10.1002/prot.21458

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iue"

Categories: Large Structures | Mus musculus | Legge GB | Sen M

@@ Line 1: / Line 1: @@
-==PACTOLUS I-DOMAIN: FUNCTIONAL SWITCHING OF THE ROSSMANN FOLD==
-<StructureSection load='2iue' size='340' side='right' caption='[[2iue]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+==Pactolus I-domain: Functional Switching of the Rossmann Fold==
+<StructureSection load='2iue' size='340' side='right'caption='[[2iue]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2iue]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IUE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2iue]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IUE FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iue OCA], [http://pdbe.org/2iue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iue RCSB], [http://www.ebi.ac.uk/pdbsum/2iue PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iue OCA], [https://pdbe.org/2iue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iue RCSB], [https://www.ebi.ac.uk/pdbsum/2iue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iue ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ITB2L_MOUSE ITB2L_MOUSE]] During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.<ref>PMID:16836649</ref>
+[https://www.uniprot.org/uniprot/ITB2L_MOUSE ITB2L_MOUSE] During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.<ref>PMID:16836649</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iue_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iue_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iue ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 30: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Legge, G B]]
+[[Category: Mus musculus]]
-[[Category: Sen, M]]
+[[Category: Legge GB]]
-[[Category: Admida]]
+[[Category: Sen M]]
-[[Category: Cd]]
-[[Category: Cell adhesion]]
-[[Category: Integrin]]
-[[Category: Itc]]
-[[Category: Limb]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Mida]]
-[[Category: Rossmann fold]]
-[[Category: Titration]]
-[[Category: Transmembrane]]

2iue

From Proteopedia

Current revision

Pactolus I-domain: Functional Switching of the Rossmann Fold

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox