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2iue

From Proteopedia

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Pactolus I-domain: Functional Switching of the Rossmann Fold

Structural highlights

2iue is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ITB2L_MOUSE During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Murine Pactolus is a neutrophil-specific single chain glycoprotein that plays a role as an apoptosis marker for macrophages. The extracellular region of the protein shows strong sequence similarities to integrin beta-subunits. Critical sequence modifications differentiate its function when compared to the integrin family. We show experimentally that Pactolus I-domain does not bind divalent metal ions, indicating that ligand binding is not mediated through a metal ion-dependent adhesion site (MIDAS). NMR data was used to map secondary structure and the strand pairing within the beta-sheet to confirm an overall Rossmann fold topology. Homology modeling enhanced by the NMR data was used to determine the overall structure, with two key loop insertions/deletions (insertion 2 and SDL) that distinguish the Pactolus I-domain from the integrin alpha I-domain and beta I-domains. NMR peak exchange broadening is observed due to dimerization, correlating to the beta I-domain and beta propeller heterodimerization region within the integrin headpiece. Two unique N-linked glycosylation sites (Asn151 and Asn230) within this region disrupt dimerization and may account for why Pactolus is not found to associate with an alpha-subunit. These changes in quaternary structure, ligand binding loops, glycosylation, and metal sites illustrate how evolution has rapidly and effectively altered key aspects of the integrin beta-subunit to derive a protein of novel function on an existing protein scaffold.

Pactolus I-domain: functional switching of the Rossmann fold.,Sen M, Legge GB Proteins. 2007 Aug 15;68(3):626-35. PMID:17523188^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hojgaard A, Close R, Dunn DM, Weiss RB, Weis JJ, Weis JH. Altered localization of CXCL13 expressing cells in mice deficient in Pactolus following an inflammatory stimulus. Immunology. 2006 Oct;119(2):212-23. Epub 2006 Jul 12. PMID:16836649 doi:http://dx.doi.org/IMM2426
↑ Sen M, Legge GB. Pactolus I-domain: functional switching of the Rossmann fold. Proteins. 2007 Aug 15;68(3):626-35. PMID:17523188 doi:10.1002/prot.21458

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iue"

Categories: Large Structures | Mus musculus | Legge GB | Sen M

@@ Line 1: / Line 1: @@
-==PACTOLUS I-DOMAIN: FUNCTIONAL SWITCHING OF THE ROSSMANN FOLD==
-<StructureSection load='2iue' size='340' side='right' caption='[[2iue]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+==Pactolus I-domain: Functional Switching of the Rossmann Fold==
+<StructureSection load='2iue' size='340' side='right'caption='[[2iue]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2iue]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IUE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2iue]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IUE FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iue OCA], [http://pdbe.org/2iue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iue RCSB], [http://www.ebi.ac.uk/pdbsum/2iue PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iue OCA], [https://pdbe.org/2iue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iue RCSB], [https://www.ebi.ac.uk/pdbsum/2iue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iue ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ITB2L_MOUSE ITB2L_MOUSE]] During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.<ref>PMID:16836649</ref>
+[https://www.uniprot.org/uniprot/ITB2L_MOUSE ITB2L_MOUSE] During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.<ref>PMID:16836649</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iue_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iue_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 30: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Legge, G B]]
+[[Category: Mus musculus]]
-[[Category: Sen, M]]
+[[Category: Legge GB]]
-[[Category: Admida]]
+[[Category: Sen M]]
-[[Category: Cd]]
-[[Category: Cell adhesion]]
-[[Category: Integrin]]
-[[Category: Itc]]
-[[Category: Limb]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Mida]]
-[[Category: Rossmann fold]]
-[[Category: Titration]]
-[[Category: Transmembrane]]

2iue

From Proteopedia

Current revision

Pactolus I-domain: Functional Switching of the Rossmann Fold

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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