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2kbt

From Proteopedia

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[[Image:2kbt.png|left|200px]]
 
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{{STRUCTURE_2kbt| PDB=2kbt | SCENE= }}
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==Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method==
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<StructureSection load='2kbt' size='340' side='right'caption='[[2kbt]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2kbt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus], [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G'] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KBT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kbt OCA], [https://pdbe.org/2kbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kbt RCSB], [https://www.ebi.ac.uk/pdbsum/2kbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kbt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.[https://www.uniprot.org/uniprot/VAV_MOUSE VAV_MOUSE] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kbt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kbt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sample solubility is essential for structural studies of proteins by solution NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and thioredoxin, to a target protein has been used for this purpose. However, signal overlap of the tag with the target protein often made the spectral analysis difficult. Here we report a sortase-mediated protein ligation method to eliminate NMR signals arising from the tag by preparing the isotopically labeled target protein attached with the non-labeled GB1 tag at the C-terminus.
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===Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method===
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Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method.,Kobashigawa Y, Kumeta H, Ogura K, Inagaki F J Biomol NMR. 2009 Jan 13. PMID:19140010<ref>PMID:19140010</ref>
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{{ABSTRACT_PUBMED_19140010}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2kbt" style="background-color:#fffaf0;"></div>
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[[2kbt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBT OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019140010</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Inagaki, F.]]
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[[Category: Streptococcus sp. 'group G']]
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[[Category: Kobashigawa, Y.]]
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[[Category: Synthetic construct]]
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[[Category: Kumeta, H.]]
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[[Category: Inagaki F]]
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[[Category: Ogura, K.]]
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[[Category: Kobashigawa Y]]
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[[Category: Gb1]]
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[[Category: Kumeta H]]
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[[Category: Inset]]
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[[Category: Ogura K]]
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[[Category: Intein]]
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[[Category: Protein ligation]]
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[[Category: Signaling protein]]
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[[Category: Solubility enhancement]]
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[[Category: Sortase]]
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Current revision

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

PDB ID 2kbt

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