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2lxa

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[[Image:2lxa.png|left|200px]]
 
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{{STRUCTURE_2lxa| PDB=2lxa | SCENE= }}
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==Solution structure of the Get5 ubiquitin-like domain==
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<StructureSection load='2lxa' size='340' side='right'caption='[[2lxa]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2lxa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LXA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxa OCA], [https://pdbe.org/2lxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lxa RCSB], [https://www.ebi.ac.uk/pdbsum/2lxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the cytoplasm, the correct delivery of membrane proteins is an essential and highly regulated process. The posttranslational targeting of the important tail-anchor membrane (TA) proteins has recently been under intense investigation. A specialized pathway, called the guided entry of TA proteins (GET) pathway in yeast and the transmembrane domain recognition complex (TRC) pathway in vertebrates, recognizes endoplasmic-reticulum-targeted TA proteins and delivers them through a complex series of handoffs. An early step is the formation of a complex between Sgt2/SGTA, a cochaperone with a presumed ubiquitin-like-binding domain (UBD), and Get5/UBL4A, a ubiquitin-like domain (UBL)-containing protein. We structurally characterize this UBD/UBL interaction for both yeast and human proteins. This characterization is supported by biophysical studies that demonstrate that complex formation is mediated by electrostatics, generating an interface that has high-affinity with rapid kinetics. In total, this work provides a refined model of the interplay of Sgt2 homologs in TA targeting.
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===Solution structure of the Get5 ubiquitin-like domain===
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Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface.,Chartron JW, Vandervelde DG, Clemons WM Jr Cell Rep. 2012 Nov 7. pii: S2211-1247(12)00346-4. doi:, 10.1016/j.celrep.2012.10.010. PMID:23142665<ref>PMID:23142665</ref>
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{{ABSTRACT_PUBMED_23142665}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2lxa" style="background-color:#fffaf0;"></div>
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[[2lxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXA OCA].
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== References ==
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[[Category: Saccharomyces cerevisiae s288c]]
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<references/>
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[[Category: Chartron, J W.]]
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__TOC__
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[[Category: Clemons, W M.]]
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</StructureSection>
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[[Category: Vandervelde, D G.]]
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[[Category: Large Structures]]
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[[Category: Get pathway]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Protein binding]]
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[[Category: Chartron JW]]
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[[Category: Protein-protein interaction]]
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[[Category: Clemons Jr WM]]
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[[Category: Sgt2 binding domain]]
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[[Category: Vandervelde DG]]
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[[Category: Ubiquitin-like domain]]
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Current revision

Solution structure of the Get5 ubiquitin-like domain

PDB ID 2lxa

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