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| | ==Structure of C-terminal domain of Ska1== | | ==Structure of C-terminal domain of Ska1== |
| - | <StructureSection load='2lyc' size='340' side='right' caption='[[2lyc]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lyc' size='340' side='right'caption='[[2lyc]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lyc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LYC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LYC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lyc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LYC FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Y106G6H.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lyc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lyc RCSB], [http://www.ebi.ac.uk/pdbsum/2lyc PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lyc OCA], [https://pdbe.org/2lyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lyc RCSB], [https://www.ebi.ac.uk/pdbsum/2lyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lyc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SKA1_CAEEL SKA1_CAEEL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2lyc" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Caenorhabditis elegans]] | | [[Category: Caenorhabditis elegans]] |
| - | [[Category: Arthanari, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Boeszoermenyi, A]] | + | [[Category: Arthanari H]] |
| - | [[Category: Cheeseman, I M]] | + | [[Category: Boeszoermenyi A]] |
| - | [[Category: Markus, M]] | + | [[Category: Cheeseman IM]] |
| - | [[Category: Oberer, M]] | + | [[Category: Markus M]] |
| - | [[Category: Schmidt, J C]] | + | [[Category: Oberer M]] |
| - | [[Category: Wagner, G]] | + | [[Category: Schmidt JC]] |
| - | [[Category: Kinetochore]]
| + | [[Category: Wagner G]] |
| - | [[Category: Microtubule]]
| + | |
| - | [[Category: Protein binding]]
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| - | [[Category: Ska1]]
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| - | [[Category: Ska1-mtbd]]
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| Structural highlights
Function
SKA1_CAEEL
Publication Abstract from PubMed
To ensure equal chromosome segregation during mitosis, the macromolecular kinetochore must remain attached to depolymerizing microtubules, which drive chromosome movements. How kinetochores associate with depolymerizing microtubules, which undergo dramatic structural changes forming curved protofilaments, has yet to be defined in vertebrates. Here, we demonstrate that the conserved kinetochore-localized Ska1 complex tracks with depolymerizing microtubule ends and associates with both the microtubule lattice and curved protofilaments. In contrast, the Ndc80 complex, a central player in the kinetochore-microtubule interface, binds only to the straight microtubule lattice and lacks tracking activity. We demonstrate that the Ska1 complex imparts its tracking capability to the Ndc80 complex. Finally, we present a structure of the Ska1 microtubule-binding domain that reveals its interaction with microtubules and its regulation by Aurora B. This work defines an integrated kinetochore-microtubule interface formed by the Ska1 and Ndc80 complexes that associates with depolymerizing microtubules, potentially by interacting with curved microtubule protofilaments.
The Kinetochore-Bound Ska1 Complex Tracks Depolymerizing Microtubules and Binds to Curved Protofilaments.,Schmidt JC, Arthanari H, Boeszoermenyi A, Dashkevich NM, Wilson-Kubalek EM, Monnier N, Markus M, Oberer M, Milligan RA, Bathe M, Wagner G, Grishchuk EL, Cheeseman IM Dev Cell. 2012 Oct 17. pii: S1534-5807(12)00423-6. doi:, 10.1016/j.devcel.2012.09.012. PMID:23085020[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schmidt JC, Arthanari H, Boeszoermenyi A, Dashkevich NM, Wilson-Kubalek EM, Monnier N, Markus M, Oberer M, Milligan RA, Bathe M, Wagner G, Grishchuk EL, Cheeseman IM. The Kinetochore-Bound Ska1 Complex Tracks Depolymerizing Microtubules and Binds to Curved Protofilaments. Dev Cell. 2012 Oct 17. pii: S1534-5807(12)00423-6. doi:, 10.1016/j.devcel.2012.09.012. PMID:23085020 doi:http://dx.doi.org/10.1016/j.devcel.2012.09.012
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