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2mwh

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Current revision

NMR solution structure of ligand-free OAA

Structural highlights

2mwh is a 1 chain structure with sequence from Planktothrix agardhii. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LEC1_PLAAG Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated rabbit erythrocytes. Does not require divalent cations for activity. Inhibits HIV replication in MT4 cells with an EC(50) of 45 nM. Binds to the HIV envelope glycoprotein gp120.^[1] ^[2]

Publication Abstract from PubMed

Lectins from different sources have been shown to interfere with HIV infection by binding to the sugars of viral-envelope glycoproteins. Three-dimensional atomic structures of a number of HIV-inactivating lectins have been determined, both as free proteins and in glycan-bound forms. However, details on the mechanism of recognition and binding to sugars are elusive. Herein we focus on the anti-HIV lectin OAA from Oscillatoria agardhii: We show that in the absence of sugars in solution, both the sugar-free and sugar-bound protein conformations that were observed in the X-ray crystal structures exist as conformational substates. Our results suggest that glycan recognition occurs by conformational selection within the ground state; this model differs from the popular "excited-state" model. Our findings provide further insight into molecular recognition of the major receptor on the HIV virus by OAA. These details can potentially be used for the optimization and/or development of preventive anti-HIV therapeutics.

Sampling of Glycan-Bound Conformers by the Anti-HIV Lectin Oscillatoria agardhii agglutinin in the Absence of Sugar.,Carneiro MG, Koharudin LM, Ban D, Sabo TM, Trigo-Mourino P, Mazur A, Griesinger C, Gronenborn AM, Lee D Angew Chem Int Ed Engl. 2015 Apr 14. doi: 10.1002/anie.201500213. PMID:25873445^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sato Y, Murakami M, Miyazawa K, Hori K. Purification and characterization of a novel lectin from a freshwater cyanobacterium, Oscillatoria agardhii. Comp Biochem Physiol B Biochem Mol Biol. 2000 Feb;125(2):169-77. PMID:10817903 doi:10.1016/s0305-0491(99)00164-9
↑ Sato Y, Okuyama S, Hori K. Primary structure and carbohydrate binding specificity of a potent anti-HIV lectin isolated from the filamentous cyanobacterium Oscillatoria agardhii. J Biol Chem. 2007 Apr 13;282(15):11021-9. Epub 2007 Feb 21. PMID:17314091 doi:10.1074/jbc.M701252200
↑ Carneiro MG, Koharudin LM, Ban D, Sabo TM, Trigo-Mourino P, Mazur A, Griesinger C, Gronenborn AM, Lee D. Sampling of Glycan-Bound Conformers by the Anti-HIV Lectin Oscillatoria agardhii agglutinin in the Absence of Sugar. Angew Chem Int Ed Engl. 2015 Apr 14. doi: 10.1002/anie.201500213. PMID:25873445 doi:http://dx.doi.org/10.1002/anie.201500213

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mwh"

@@ Line 1: / Line 1: @@
 ==NMR solution structure of ligand-free OAA==
-<StructureSection load='2mwh' size='340' side='right' caption='[[2mwh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2mwh' size='340' side='right'caption='[[2mwh]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mwh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"planktothrix_agardhii"_emend._suda_et_al._2002 "planktothrix agardhii" emend. suda et al. 2002]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MWH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MWH FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwh OCA], [http://pdbe.org/2mwh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mwh RCSB], [http://www.ebi.ac.uk/pdbsum/2mwh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwh ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwh OCA], [https://pdbe.org/2mwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwh RCSB], [https://www.ebi.ac.uk/pdbsum/2mwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwh ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LEC1_PLAAG LEC1_PLAAG] Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated rabbit erythrocytes. Does not require divalent cations for activity. Inhibits HIV replication in MT4 cells with an EC(50) of 45 nM. Binds to the HIV envelope glycoprotein gp120.<ref>PMID:10817903</ref> <ref>PMID:17314091</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Planktothrix agardhii emend. suda et al. 2002]]
+[[Category: Large Structures]]
-[[Category: Ban, D]]
+[[Category: Planktothrix agardhii]]
-[[Category: Carneiro, M G]]
+[[Category: Ban D]]
-[[Category: Griesinger, C]]
+[[Category: Carneiro MG]]
-[[Category: Gronenborn, A M]]
+[[Category: Griesinger C]]
-[[Category: Koharudin, L M]]
+[[Category: Gronenborn AM]]
-[[Category: Lee, D]]
+[[Category: Koharudin LM]]
-[[Category: Mazur, A]]
+[[Category: Lee D]]
-[[Category: Sabo, T]]
+[[Category: Mazur A]]
-[[Category: Trigo-Mourino, P]]
+[[Category: Sabo T]]
-[[Category: Protein binding]]
+[[Category: Trigo-Mourino P]]
-[[Category: Sugar binding protein-antiviral protein complex]]

2mwh

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Current revision

NMR solution structure of ligand-free OAA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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