1y0j

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[[Image:1y0j.gif|left|200px]]
 
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{{Structure
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==Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction==
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|PDB= 1y0j |SIZE=350|CAPTION= <scene name='initialview01'>1y0j</scene>
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<StructureSection load='1y0j' size='340' side='right'caption='[[1y0j]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1y0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The March 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Zinc Fingers'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_3 10.2210/rcsb_pdb/mom_2007_3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y0J FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= gata-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), u-shaped ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y0j OCA], [https://pdbe.org/1y0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y0j RCSB], [https://www.ebi.ac.uk/pdbsum/1y0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y0j ProSAT]</span></td></tr>
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</table>
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'''Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction'''
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== Function ==
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[https://www.uniprot.org/uniprot/GATA1_MOUSE GATA1_MOUSE] Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.<ref>PMID:2276623</ref> <ref>PMID:8206977</ref> <ref>PMID:8524811</ref> <ref>PMID:15173587</ref> <ref>PMID:16888089</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y0/1y0j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y0j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
GATA-1 and friend of GATA (FOG) are zinc-finger transcription factors that physically interact to play essential roles in erythroid and megakaryocytic development. Several naturally occurring mutations in the GATA-1 gene that alter the FOG-binding domain have been reported. The mutations are associated with familial anemias and thrombocytopenias of differing severity. To elucidate the molecular basis for the GATA-1/FOG interaction, we have determined the three-dimensional structure of a complex comprising the interaction domains of these proteins. The structure reveals how zinc fingers can act as protein recognition motifs. Details of the architecture of the contact domains and their physical properties provide a molecular explanation for how the GATA-1 mutations contribute to distinct but related genetic diseases.
GATA-1 and friend of GATA (FOG) are zinc-finger transcription factors that physically interact to play essential roles in erythroid and megakaryocytic development. Several naturally occurring mutations in the GATA-1 gene that alter the FOG-binding domain have been reported. The mutations are associated with familial anemias and thrombocytopenias of differing severity. To elucidate the molecular basis for the GATA-1/FOG interaction, we have determined the three-dimensional structure of a complex comprising the interaction domains of these proteins. The structure reveals how zinc fingers can act as protein recognition motifs. Details of the architecture of the contact domains and their physical properties provide a molecular explanation for how the GATA-1 mutations contribute to distinct but related genetic diseases.
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==About this Structure==
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Zinc fingers as protein recognition motifs: structural basis for the GATA-1/friend of GATA interaction.,Liew CK, Simpson RJ, Kwan AH, Crofts LA, Loughlin FE, Matthews JM, Crossley M, Mackay JP Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):583-8. Epub 2005 Jan 11. PMID:15644435<ref>PMID:15644435</ref>
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1Y0J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The following page contains interesting information on the relation of 1Y0J with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0J OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Zinc fingers as protein recognition motifs: structural basis for the GATA-1/friend of GATA interaction., Liew CK, Simpson RJ, Kwan AH, Crofts LA, Loughlin FE, Matthews JM, Crossley M, Mackay JP, Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):583-8. Epub 2005 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15644435 15644435]
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</div>
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<div class="pdbe-citations 1y0j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Zinc Fingers]]
[[Category: Zinc Fingers]]
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[[Category: Crofts, L A.]]
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[[Category: Crofts LA]]
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[[Category: Crossley, M.]]
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[[Category: Crossley M]]
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[[Category: Kwan, A H.Y.]]
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[[Category: Kwan AHY]]
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[[Category: Liew, C K.]]
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[[Category: Liew CK]]
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[[Category: Loughlin, F E.]]
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[[Category: Loughlin FE]]
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[[Category: Mackay, J P.]]
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[[Category: Mackay JP]]
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[[Category: Matthews, J M.]]
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[[Category: Matthews JM]]
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[[Category: Simpson, R J.Y.]]
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[[Category: Simpson RJY]]
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[[Category: ZN]]
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[[Category: fog]]
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[[Category: gata-1]]
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[[Category: protein-protein complex]]
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[[Category: zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:16:57 2008''
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Current revision

Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction

PDB ID 1y0j

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