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1yhp

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[[Image:1yhp.gif|left|200px]]<br /><applet load="1yhp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yhp" />
 
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'''Solution Structure of Ca2+-free DdCAD-1'''<br />
 
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==Overview==
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==Solution Structure of Ca2+-free DdCAD-1==
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DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a, hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is, expressed by the social amoeba Dictyostelium discoideum at the onset of, development. It is synthesized as a soluble protein and then transported, to the plasma membrane by contractile vacuoles. Here we describe the novel, features of the solution structures of Ca(2+)-free and Ca(2+)-bound, monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging, to the betagamma-crystallin and immunoglobulin fold classes, respectively., Whereas the N-terminal domain has a major role in homophilic binding, the, C-terminal domain tethers the protein to the cell membrane. From, structural and mutational analyses, we propose a model for the, Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated, cell-cell adhesion at the molecular level. Our results provide new, insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.
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<StructureSection load='1yhp' size='340' side='right'caption='[[1yhp]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1yhp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YHP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yhp OCA], [https://pdbe.org/1yhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yhp RCSB], [https://www.ebi.ac.uk/pdbsum/1yhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yhp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAD1_DICDI CAD1_DICDI] Mediates calcium-dependent cell-cell adhesion during the early stage of development.<ref>PMID:8663243</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yh/1yhp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yhp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is expressed by the social amoeba Dictyostelium discoideum at the onset of development. It is synthesized as a soluble protein and then transported to the plasma membrane by contractile vacuoles. Here we describe the novel features of the solution structures of Ca(2+)-free and Ca(2+)-bound monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging to the betagamma-crystallin and immunoglobulin fold classes, respectively. Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane. From structural and mutational analyses, we propose a model for the Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated cell-cell adhesion at the molecular level. Our results provide new insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.
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==About this Structure==
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Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion.,Lin Z, Sriskanthadevan S, Huang H, Siu CH, Yang D Nat Struct Mol Biol. 2006 Nov;13(11):1016-22. Epub 2006 Oct 22. PMID:17057715<ref>PMID:17057715</ref>
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1YHP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YHP OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion., Lin Z, Sriskanthadevan S, Huang H, Siu CH, Yang D, Nat Struct Mol Biol. 2006 Nov;13(11):1016-22. Epub 2006 Oct 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17057715 17057715]
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</div>
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<div class="pdbe-citations 1yhp" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Huang, H.B.]]
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[[Category: Huang HB]]
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[[Category: Lin, Z.]]
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[[Category: Lin Z]]
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[[Category: Siu, C.H.]]
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[[Category: Siu CH]]
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[[Category: Yang, D.W.]]
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[[Category: Yang DW]]
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[[Category: calcium]]
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[[Category: cell adhesion]]
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[[Category: ddcad-1]]
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[[Category: nmr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:17:19 2008''
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Current revision

Solution Structure of Ca2+-free DdCAD-1

PDB ID 1yhp

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