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1z24

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[[Image:1z24.gif|left|200px]]
 
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==The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.==
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The line below this paragraph, containing "STRUCTURE_1z24", creates the "Structure Box" on the page.
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<StructureSection load='1z24' size='340' side='right'caption='[[1z24]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1z24]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z24 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLV:BILIVERDIN+IX+GAMMA+CHROMOPHORE'>BLV</scene></td></tr>
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{{STRUCTURE_1z24| PDB=1z24 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z24 OCA], [https://pdbe.org/1z24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z24 RCSB], [https://www.ebi.ac.uk/pdbsum/1z24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z24 ProSAT]</span></td></tr>
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</table>
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'''The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.'''
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== Function ==
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[https://www.uniprot.org/uniprot/ICYA_MANSE ICYA_MANSE] This protein binds a chromophore: biliverdin IX, isomer gamma. Mixed with lipoprotein-bound carotenes, this blue protein provides hornworms with their green cryptic coloration which serves a camouflage.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/1z24_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z24 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Insecticyanin, a blue biliprotein isolated from the tobacco hornworm Manduca sexta L., is involved in insect camouflage. Its three-dimensional structure has now been solved to 2.6 A resolution using the techniques of multiple isomorphous replacement, non-crystallographic symmetry averaging about a local 2-fold rotation axis and solvent flattening. All 189 amino acids have been fitted to the electron density map. The map clearly shows that insecticyanin is a tetramer with one of its molecular 2-fold axes coincident to a crystallographic dyad. The individual subunits have overall dimensions of 44 A X 37 A X 40 A and consist primarily of an eight-stranded anti-parallel beta-barrel flanked on one side by a 4.5-turn alpha-helix. Interestingly the overall three-dimensional fold of the insecticyanin subunit shows remarkable similarity to the structural motifs of bovine beta-lactoglobulin and the human serum retinol-binding protein. The electron density attributable to the chromophore is unambiguous and shows that it is indeed the gamma-isomer of biliverdin. The biliverdin lies towards the open end of the beta-barrel with its two propionate side chains pointing towards the solvent and it adopts a rather folded conformation, much like a heme.
Insecticyanin, a blue biliprotein isolated from the tobacco hornworm Manduca sexta L., is involved in insect camouflage. Its three-dimensional structure has now been solved to 2.6 A resolution using the techniques of multiple isomorphous replacement, non-crystallographic symmetry averaging about a local 2-fold rotation axis and solvent flattening. All 189 amino acids have been fitted to the electron density map. The map clearly shows that insecticyanin is a tetramer with one of its molecular 2-fold axes coincident to a crystallographic dyad. The individual subunits have overall dimensions of 44 A X 37 A X 40 A and consist primarily of an eight-stranded anti-parallel beta-barrel flanked on one side by a 4.5-turn alpha-helix. Interestingly the overall three-dimensional fold of the insecticyanin subunit shows remarkable similarity to the structural motifs of bovine beta-lactoglobulin and the human serum retinol-binding protein. The electron density attributable to the chromophore is unambiguous and shows that it is indeed the gamma-isomer of biliverdin. The biliverdin lies towards the open end of the beta-barrel with its two propionate side chains pointing towards the solvent and it adopts a rather folded conformation, much like a heme.
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==About this Structure==
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The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.,Holden HM, Rypniewski WR, Law JH, Rayment I EMBO J. 1987 Jun;6(6):1565-70. PMID:3608987<ref>PMID:3608987</ref>
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1Z24 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z24 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution., Holden HM, Rypniewski WR, Law JH, Rayment I, EMBO J. 1987 Jun;6(6):1565-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3608987 3608987]
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</div>
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<div class="pdbe-citations 1z24" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Manduca sexta]]
[[Category: Manduca sexta]]
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[[Category: Single protein]]
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[[Category: Holden HM]]
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[[Category: Holden, H M.]]
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[[Category: Law JH]]
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[[Category: Law, J H.]]
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[[Category: Rayment I]]
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[[Category: Rayment, I.]]
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[[Category: Rypniewski WR]]
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[[Category: Rypniewski, W R.]]
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[[Category: Blue biliprotein]]
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[[Category: Chromophore binding.]]
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[[Category: Ins-a]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:05:38 2008''
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Current revision

The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.

PDB ID 1z24

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