1z8s

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(New page: 200px<br /><applet load="1z8s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z8s" /> '''DnaB binding domain of DnaG (P16) from Bacil...)
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[[Image:1z8s.gif|left|200px]]<br /><applet load="1z8s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z8s" />
 
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'''DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)'''<br />
 
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==Overview==
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==DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)==
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The helicase-primase interaction is a critical event in DNA replication, and is mediated by a putative helicase-interaction domain within the, primase. The solution structure of the helicase-interaction domain of DnaG, reveals that it is made up of two independent subdomains: an N-terminal, six-helix module and a C-terminal two-helix module that contains the, residues of the primase previously identified as important in the, interaction with the helicase. We show that the two-helix module alone is, sufficient for strong binding between the primase and the helicase but, fails to activate the helicase; both subdomains are required for helicase, activation. The six-helix module of the primase has only one close, structural homolog, the N-terminal domain of the corresponding helicase., This surprising structural relationship, coupled with the differences in, surface properties of the two molecules, suggests how the, helicase-interaction domain may perturb the structure of the helicase and, lead to activation.
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<StructureSection load='1z8s' size='340' side='right'caption='[[1z8s]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z8s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z8S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z8s OCA], [https://pdbe.org/1z8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z8s RCSB], [https://www.ebi.ac.uk/pdbsum/1z8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z8s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNAG_GEOSE DNAG_GEOSE] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.[HAMAP-Rule:MF_00974]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/1z8s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z8s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The helicase-primase interaction is a critical event in DNA replication and is mediated by a putative helicase-interaction domain within the primase. The solution structure of the helicase-interaction domain of DnaG reveals that it is made up of two independent subdomains: an N-terminal six-helix module and a C-terminal two-helix module that contains the residues of the primase previously identified as important in the interaction with the helicase. We show that the two-helix module alone is sufficient for strong binding between the primase and the helicase but fails to activate the helicase; both subdomains are required for helicase activation. The six-helix module of the primase has only one close structural homolog, the N-terminal domain of the corresponding helicase. This surprising structural relationship, coupled with the differences in surface properties of the two molecules, suggests how the helicase-interaction domain may perturb the structure of the helicase and lead to activation.
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==About this Structure==
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Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation.,Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP Structure. 2005 Apr;13(4):609-16. PMID:15837199<ref>PMID:15837199</ref>
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1Z8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z8S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15837199 15837199]
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</div>
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[[Category: Geobacillus stearothermophilus]]
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<div class="pdbe-citations 1z8s" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Hounslow, A.M.]]
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[[Category: Soultanas, P.]]
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[[Category: Syson, K.]]
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[[Category: Thirlway, J.]]
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[[Category: Waltho, J.P.]]
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[[Category: two alpha helical sub-domains]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:18:20 2007''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Large Structures]]
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[[Category: Hounslow AM]]
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[[Category: Soultanas P]]
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[[Category: Syson K]]
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[[Category: Thirlway J]]
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[[Category: Waltho JP]]

Current revision

DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)

PDB ID 1z8s

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