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1zlb

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{{Seed}}
 
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[[Image:1zlb.png|left|200px]]
 
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==Crystal structure of catalytically-active phospholipase A2 in the absence of calcium==
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The line below this paragraph, containing "STRUCTURE_1zlb", creates the "Structure Box" on the page.
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<StructureSection load='1zlb' size='340' side='right'caption='[[1zlb]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zlb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZLB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlb OCA], [https://pdbe.org/1zlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zlb RCSB], [https://www.ebi.ac.uk/pdbsum/1zlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zlb ProSAT]</span></td></tr>
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{{STRUCTURE_1zlb| PDB=1zlb | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA2A_BOTJR PA2A_BOTJR] Snake venom phospholipase A2 (PLA2) that induces edema (activity that is inhibited by EDTA and dexamethasone), inhibits phospholipid-dependent collagen/ADP-induced platelet aggregation, possess hypotensive as well as anticoagulant activities. In addition, this enzyme shows bactericidal activity against E.coli and S.aureus. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:12167491</ref> <ref>PMID:15214498</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zl/1zlb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zlb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The electrophile Ca(2+) is an essential multifunctional co-factor in the phospholipase A(2) mediated hydrolysis of phospholipids. Crystal structures of an acidic phospholipase A(2) from the venom of Bothrops jararacussu have been determined both in the Ca(2+) free and bound states at 0.97 and 1.60 A resolutions, respectively. In the Ca(2+) bound state, the Ca(2+) ion is penta-coordinated by a distorted pyramidal cage of oxygen and nitrogen atoms that is significantly different to that observed in structures of other Group I/II phospholipases A(2). In the absence of Ca(2+), a water molecule occupies the position of the Ca(2+) ion and the side chain of Asp49 and the calcium-binding loop adopts a different conformation.
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===Crystal structure of catalytically-active phospholipase A2 in the absence of calcium===
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Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states.,Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:16376474<ref>PMID:16376474</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1zlb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16376474}}, adds the Publication Abstract to the page
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16376474 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16376474}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1ZLB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLB OCA].
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==Reference==
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Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states., Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK, Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16376474 16376474]
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Crystallization and high-resolution X-ray diffraction data collection of an Asp49 PLA2 from Bothrops jararacussu venom both in the presence and absence of Ca2+ ions., Murakami MT, Michelan-Duarte S, Cintra AC, Arni RK, Biochim Biophys Acta. 2004 Dec 1;1703(1):79-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15588705 15588705]
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[[Category: Bothrops jararacussu]]
[[Category: Bothrops jararacussu]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arni, R K.]]
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[[Category: Arni RK]]
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[[Category: Betzel, C.]]
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[[Category: Betzel C]]
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[[Category: Cintra, A C.]]
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[[Category: Cintra AC]]
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[[Category: Gabdoulkhakov, A.]]
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[[Category: Gabdoulkhakov A]]
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[[Category: Genov, N.]]
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[[Category: Genov N]]
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[[Category: Murakami, M T.]]
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[[Category: Murakami MT]]
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[[Category: Asp49-pla2]]
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[[Category: Snake venom]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:18:19 2008''
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Current revision

Crystal structure of catalytically-active phospholipase A2 in the absence of calcium

PDB ID 1zlb

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