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1zun

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(New page: 200px<br /><applet load="1zun" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zun, resolution 2.70&Aring;" /> '''Crystal Structure of...)
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[[Image:1zun.gif|left|200px]]<br /><applet load="1zun" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zun, resolution 2.70&Aring;" />
 
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'''Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae'''<br />
 
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==Overview==
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==Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae==
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Sulfate assimilation is a critical component of both primary and secondary, metabolism. An essential step in this pathway is the activation of sulfate, through adenylation by the enzyme ATP sulfurylase (ATPS), forming, adenosine 5'-phosphosulfate (APS). Proteobacterial ATPS overcomes this, energetically unfavorable reaction by associating with a regulatory G, protein, coupling the energy of GTP hydrolysis to APS formation. To, discover the molecular basis of this unusual role for a G protein, we, biochemically characterized and solved the X-ray crystal structure of a, complex between Pseudomonas syringae ATPS (CysD) and its associated, regulatory G protein (CysN). The structure of CysN*D shows the two, proteins in tight association; however, the nucleotides bound to each, subunit are spatially segregated. We provide evidence that conserved, switch motifs in the G domain of CysN allosterically mediate interactions, between the nucleotide binding sites. This structure suggests a molecular, mechanism by which conserved G domain architecture is used to, energetically link GTP turnover to the production of an essential, metabolite.
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<StructureSection load='1zun' size='340' side='right'caption='[[1zun]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zun]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae] and [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._tomato_str._DC3000 Pseudomonas syringae pv. tomato str. DC3000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZUN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zun OCA], [https://pdbe.org/1zun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zun RCSB], [https://www.ebi.ac.uk/pdbsum/1zun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zun ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSD_PSESM CYSD_PSESM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zu/1zun_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zun ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sulfate assimilation is a critical component of both primary and secondary metabolism. An essential step in this pathway is the activation of sulfate through adenylation by the enzyme ATP sulfurylase (ATPS), forming adenosine 5'-phosphosulfate (APS). Proteobacterial ATPS overcomes this energetically unfavorable reaction by associating with a regulatory G protein, coupling the energy of GTP hydrolysis to APS formation. To discover the molecular basis of this unusual role for a G protein, we biochemically characterized and solved the X-ray crystal structure of a complex between Pseudomonas syringae ATPS (CysD) and its associated regulatory G protein (CysN). The structure of CysN*D shows the two proteins in tight association; however, the nucleotides bound to each subunit are spatially segregated. We provide evidence that conserved switch motifs in the G domain of CysN allosterically mediate interactions between the nucleotide binding sites. This structure suggests a molecular mechanism by which conserved G domain architecture is used to energetically link GTP turnover to the production of an essential metabolite.
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==About this Structure==
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Molecular basis for G protein control of the prokaryotic ATP sulfurylase.,Mougous JD, Lee DH, Hubbard SC, Schelle MW, Vocadlo DJ, Berger JM, Bertozzi CR Mol Cell. 2006 Jan 6;21(1):109-22. PMID:16387658<ref>PMID:16387658</ref>
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1ZUN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae] and [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._tomato_str._dc3000 Pseudomonas syringae pv. tomato str. dc3000] with MG, NA, GDP and AGS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZUN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Molecular basis for G protein control of the prokaryotic ATP sulfurylase., Mougous JD, Lee DH, Hubbard SC, Schelle MW, Vocadlo DJ, Berger JM, Bertozzi CR, Mol Cell. 2006 Jan 6;21(1):109-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16387658 16387658]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1zun" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas syringae]]
[[Category: Pseudomonas syringae]]
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[[Category: Pseudomonas syringae pv. tomato str. dc3000]]
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[[Category: Pseudomonas syringae pv. tomato str. DC3000]]
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[[Category: Sulfate adenylyltransferase]]
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[[Category: Berger JM]]
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[[Category: Berger, J.M.]]
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[[Category: Bertozzi CR]]
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[[Category: Bertozzi, C.R.]]
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[[Category: Hubbard SC]]
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[[Category: Hubbard, S.C.]]
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[[Category: Lee DH]]
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[[Category: Lee, D.H.]]
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[[Category: Mougous JD]]
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[[Category: Mougous, J.D.]]
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[[Category: Schelle MW]]
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[[Category: Schelle, M.W.]]
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[[Category: Vocadlo DJ]]
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[[Category: Vocadlo, D.J.]]
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[[Category: AGS]]
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[[Category: GDP]]
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[[Category: MG]]
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[[Category: NA]]
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[[Category: beta barrel]]
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[[Category: g protein]]
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[[Category: gtpase]]
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[[Category: heterodimer]]
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[[Category: pyrophosphate]]
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[[Category: switch domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:40:21 2007''
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Current revision

Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae

PDB ID 1zun

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