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| | ==THE NMR SOLUTION STRUCTURE OF THE HOMEODOMAIN OF THE RAT INSULIN GENE ENHANCER PROTEIN ISL-1, 50 STRUCTURES== | | ==THE NMR SOLUTION STRUCTURE OF THE HOMEODOMAIN OF THE RAT INSULIN GENE ENHANCER PROTEIN ISL-1, 50 STRUCTURES== |
| - | <StructureSection load='1bw5' size='340' side='right'caption='[[1bw5]], [[NMR_Ensembles_of_Models | 50 NMR models]]' scene=''> | + | <StructureSection load='1bw5' size='340' side='right'caption='[[1bw5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1bw5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BW5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1bw5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BW5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bw5 OCA], [http://pdbe.org/1bw5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bw5 RCSB], [http://www.ebi.ac.uk/pdbsum/1bw5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bw5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bw5 OCA], [https://pdbe.org/1bw5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bw5 RCSB], [https://www.ebi.ac.uk/pdbsum/1bw5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bw5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ISL2_RAT ISL2_RAT]] Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity). | + | [https://www.uniprot.org/uniprot/ISL2_RAT ISL2_RAT] Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Rattus norvegicus]] | | [[Category: Rattus norvegicus]] |
| - | [[Category: Behravan, G]] | + | [[Category: Behravan G]] |
| - | [[Category: Ippel, J H]] | + | [[Category: Ippel JH]] |
| - | [[Category: Larsson, G]] | + | [[Category: Larsson G]] |
| - | [[Category: Lundqvist, M]] | + | [[Category: Lundqvist M]] |
| - | [[Category: Lycksell, P O]] | + | [[Category: Lycksell P-O]] |
| - | [[Category: Schleucher, J]] | + | [[Category: Schleucher J]] |
| - | [[Category: Wijmenga, S S]] | + | [[Category: Wijmenga SS]] |
| - | [[Category: Zdunek, J]] | + | [[Category: Zdunek J]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna-binding protein]]
| + | |
| - | [[Category: Homeodomain]]
| + | |
| - | [[Category: Lim domain]]
| + | |
| Structural highlights
Function
ISL2_RAT Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Homeodomains are one of the key families of eukaryotic DNA-binding motifs and provide an important model system for DNA recognition. We have determined a high-quality nuclear magnetic resonance (NMR) structure of the DNA-binding homeodomain of the insulin gene enhancer protein Isl-1 (Isl-1-HD). It forms the first solution structure of a homeodomain from the LIM family. It contains a well-defined inner core (residues 12-55) consisting of the classical three-helix structure observed in other homeodomains. The N terminus is unstructured up to residue 8, while the C terminus gradually becomes unstructured from residue 55 onwards. Some flexibility is evident in the loop parts of the inner core. Isl-1-HD has, despite its low sequence identity (23-34 %), a structure that is strikingly similar to that of the other homeodomains with known three-dimensional structures. Detailed analysis of Isl-1-HD and the other homeodomains rationalizes the differences in their temperature stability and explains the low stability of the Isl-1-HD in the free state (tm 22-30 degrees C). Upon DNA binding, a significant stabilization occurs (tm>55 degrees C). The low stability of Isl-1-HD (and other mammalian homeodomains) suggests that in vivo Isl-1-HD recognizes its cognate DNA from its unfolded state.
The solution structure of the homeodomain of the rat insulin-gene enhancer protein isl-1. Comparison with other homeodomains.,Ippel H, Larsson G, Behravan G, Zdunek J, Lundqvist M, Schleucher J, Lycksell PO, Wijmenga S J Mol Biol. 1999 May 14;288(4):689-703. PMID:10329173[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ippel H, Larsson G, Behravan G, Zdunek J, Lundqvist M, Schleucher J, Lycksell PO, Wijmenga S. The solution structure of the homeodomain of the rat insulin-gene enhancer protein isl-1. Comparison with other homeodomains. J Mol Biol. 1999 May 14;288(4):689-703. PMID:10329173 doi:10.1006/jmbi.1999.2718
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