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| | ==STAPHYLOCOCCAL PROTEIN A E-DOMAIN (-60), NMR, MINIMIZED AVERAGE STRUCTURE== | | ==STAPHYLOCOCCAL PROTEIN A E-DOMAIN (-60), NMR, MINIMIZED AVERAGE STRUCTURE== |
| - | <StructureSection load='1edk' size='340' side='right' caption='[[1edk]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='1edk' size='340' side='right'caption='[[1edk]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1edk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EDK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1edk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EDK FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1edl|1edl]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1edk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1edk OCA], [http://pdbe.org/1edk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1edk RCSB], [http://www.ebi.ac.uk/pdbsum/1edk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1edk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1edk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1edk OCA], [https://pdbe.org/1edk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1edk RCSB], [https://www.ebi.ac.uk/pdbsum/1edk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1edk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fairbrother, W J]] | + | [[Category: Large Structures]] |
| - | [[Category: Skelton, N J]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Starovasnik, M A]] | + | [[Category: Fairbrother WJ]] |
| - | [[Category: Cell wall]] | + | [[Category: Skelton NJ]] |
| - | [[Category: Igg-binding protein]] | + | [[Category: Starovasnik MA]] |
| - | [[Category: Immunoglobulin-binding protein]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| Structural highlights
Function
SPA_STAAU
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features of Fc binding by protein A, and as a first step in developing a structural understanding of E-domain/Fv complex formation, we have determined the solution structure of the uncomplexed E-domain using 2D homonuclear and heteronuclear NMR spectroscopy. Complete 1H and 15N resonance assignments were obtained, and the structure was determined from 383 NOE-derived distance restrains, 34 phi and 19 chi 1 dihedral angle restraints, and 54 restraints for 27 H-bonds. 3JH alpha-H beta coupling constants and long-range NOEs involving Phe11 indicate the side chain exists in more than one conformation with differing chi 1 values. NOE restraints that were incompatible with chi 1 = -60 degrees were removed from one set of structure calculations, and those incompatible with chi 1 = 180 degrees were removed from a second set to allow Phe11 to explore both rotamer wells. Thus, two sets of 20 final structures, having no distance or dihedral angle restraint violations greater than 0.12 A or 1.6 degrees, respectively, represent the solution structure of the E-domain. Backbone atomic rms differences with respect to the mean coordinates for each set of 20 structures for residues 8-53 averaged 0.41 +/- 0.06 and 0.35 +/- 0.06 A. No significant differences in the overall structure result from the different orientations of Phe11. The solution structure of the E-domain consists of three alpha-helices that pack together to form a compact helical bundle. A detailed comparison between the E-domain ensembles and the previously determined structure for the B-domain in complex with Fc indicates that only the 180 degrees chi 1 rotamer of Phe11 is competent for binding; the -60 degrees chi 1 rotamer must reorient to 180 degrees to create a cavity that is filled by Ile253 from the CH2 domain of Fc in the Fc-bound complex.
Solution structure of the E-domain of staphylococcal protein A.,Starovasnik MA, Skelton NJ, O'Connell MP, Kelley RF, Reilly D, Fairbrother WJ Biochemistry. 1996 Dec 3;35(48):15558-69. PMID:8952510[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Starovasnik MA, Skelton NJ, O'Connell MP, Kelley RF, Reilly D, Fairbrother WJ. Solution structure of the E-domain of staphylococcal protein A. Biochemistry. 1996 Dec 3;35(48):15558-69. PMID:8952510 doi:10.1021/bi961409x
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