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1fu6

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(New page: 200px<br /><applet load="1fu6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fu6" /> '''NMR STRUCTURE OF THE N-SH2 DOMAIN OF THE P85...)
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[[Image:1fu6.gif|left|200px]]<br /><applet load="1fu6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fu6" />
 
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'''NMR STRUCTURE OF THE N-SH2 DOMAIN OF THE P85 SUBUNIT OF PI3-KINASE'''<br />
 
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==Overview==
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==NMR STRUCTURE OF THE N-SH2 DOMAIN OF THE P85 SUBUNIT OF PI3-KINASE==
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The N-terminal src homology 2 (SH2) domain of the p85 subunit of, phosphoinositide 3-kinase (PI3K) has a higher affinity for a peptide with, two phosphotyrosines than for the same peptide with only one. This, unexpected result was not observed for the C-terminal SH2 from the same, protein. NMR structural analysis has been used to understand the behavior, of the N-SH2. The structure of the free SH2 domain has been compared to, that of the SH2 complexed with a doubly phosphorylated peptide derived, from polyomavirus middle T antigen (MT). The structure of the free SH2, domain shows some differences from previous NMR and X-ray structures. In, the N-SH2 complexed with a doubly phosphorylated peptide, a second site, for phosphotyrosine interaction has been identified. Further, line shapes, of NMR signals showed that the SH2 protein-ligand complex is subject to, temperature-dependent conformational mobility. Conformational mobility is, also supported by the spectra of the ligand peptide. A binding model which, accounts for these results is developed.
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<StructureSection load='1fu6' size='340' side='right'caption='[[1fu6]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fu6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FU6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fu6 OCA], [https://pdbe.org/1fu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fu6 RCSB], [https://www.ebi.ac.uk/pdbsum/1fu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fu6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P85A_RAT P85A_RAT] Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/1fu6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fu6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K) has a higher affinity for a peptide with two phosphotyrosines than for the same peptide with only one. This unexpected result was not observed for the C-terminal SH2 from the same protein. NMR structural analysis has been used to understand the behavior of the N-SH2. The structure of the free SH2 domain has been compared to that of the SH2 complexed with a doubly phosphorylated peptide derived from polyomavirus middle T antigen (MT). The structure of the free SH2 domain shows some differences from previous NMR and X-ray structures. In the N-SH2 complexed with a doubly phosphorylated peptide, a second site for phosphotyrosine interaction has been identified. Further, line shapes of NMR signals showed that the SH2 protein-ligand complex is subject to temperature-dependent conformational mobility. Conformational mobility is also supported by the spectra of the ligand peptide. A binding model which accounts for these results is developed.
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==About this Structure==
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NMR structure of the N-SH2 of the p85 subunit of phosphoinositide 3-kinase complexed to a doubly phosphorylated peptide reveals a second phosphotyrosine binding site.,Weber T, Schaffhausen B, Liu Y, Gunther UL Biochemistry. 2000 Dec 26;39(51):15860-9. PMID:11123912<ref>PMID:11123912</ref>
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1FU6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FU6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR structure of the N-SH2 of the p85 subunit of phosphoinositide 3-kinase complexed to a doubly phosphorylated peptide reveals a second phosphotyrosine binding site., Weber T, Schaffhausen B, Liu Y, Gunther UL, Biochemistry. 2000 Dec 26;39(51):15860-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11123912 11123912]
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</div>
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[[Category: Rattus norvegicus]]
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<div class="pdbe-citations 1fu6" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Guenther, U.L.]]
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[[Category: Liu, Y.]]
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[[Category: Schaffhausen, B.]]
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[[Category: Weber, T.]]
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[[Category: central beta-sheet with two flanking alpha-helices]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:17:17 2007''
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==See Also==
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*[[Phosphoinositide 3-kinase 3D structures|Phosphoinositide 3-kinase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rattus norvegicus]]
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[[Category: Guenther UL]]
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[[Category: Liu Y]]
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[[Category: Schaffhausen B]]
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[[Category: Weber T]]

Current revision

NMR STRUCTURE OF THE N-SH2 DOMAIN OF THE P85 SUBUNIT OF PI3-KINASE

PDB ID 1fu6

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