1jjx

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[[Image:1jjx.jpg|left|200px]]
 
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{{Structure
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==Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein==
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|PDB= 1jjx |SIZE=350|CAPTION= <scene name='initialview01'>1jjx</scene>
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<StructureSection load='1jjx' size='340' side='right'caption='[[1jjx]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1jjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJX FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjx OCA], [https://pdbe.org/1jjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjx RCSB], [https://www.ebi.ac.uk/pdbsum/1jjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjx ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein'''
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[https://www.uniprot.org/uniprot/FABP7_HUMAN FABP7_HUMAN] B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jjx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jjx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Human brain-type fatty acid-binding protein (B-FABP) has been recombinantly expressed in Escherichia coli both unlabelled and 15N-enriched for structure investigation in solution using high-resolution NMR spectroscopy. The sequential assignments of the 1H and 15N resonances were achieved by applying multidimensional homo- and heteronuclear NMR experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a total of 2490 meaningful distance constraints. The overall B-FABP structure exhibits the typical backbone conformation described for other members of the FABP family, consisting often antiparallel beta-strands (betaA to betaJ) that form two almost orthogonal beta-sheets, a helix-turn-helix motif that closes the beta-barrel on one side, and a short N-terminal helical loop. A comparison with the crystal structure of the same protein complexed with docosahexaenoic acid reveals only minor differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP and heart-type FABP with respect to fatty acid binding inside the protein cavity.
Human brain-type fatty acid-binding protein (B-FABP) has been recombinantly expressed in Escherichia coli both unlabelled and 15N-enriched for structure investigation in solution using high-resolution NMR spectroscopy. The sequential assignments of the 1H and 15N resonances were achieved by applying multidimensional homo- and heteronuclear NMR experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a total of 2490 meaningful distance constraints. The overall B-FABP structure exhibits the typical backbone conformation described for other members of the FABP family, consisting often antiparallel beta-strands (betaA to betaJ) that form two almost orthogonal beta-sheets, a helix-turn-helix motif that closes the beta-barrel on one side, and a short N-terminal helical loop. A comparison with the crystal structure of the same protein complexed with docosahexaenoic acid reveals only minor differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP and heart-type FABP with respect to fatty acid binding inside the protein cavity.
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==Disease==
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Solution structure of fatty acid-binding protein from human brain.,Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:12479569<ref>PMID:12479569</ref>
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Known diseases associated with this structure: Infundibular hypoplasia and hypopituitarism OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313430 313430]], Mental retardation, X-linked, with isolated growth hormone deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313430 313430]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1JJX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJX OCA].
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</div>
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<div class="pdbe-citations 1jjx" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Solution structure of fatty acid-binding protein from human brain., Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C, Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12479569 12479569]
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*[[Fatty acid-binding protein 3D structures|Fatty acid-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Luecke, C.]]
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[[Category: Luecke C]]
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[[Category: Rademacher, M.]]
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[[Category: Rademacher M]]
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[[Category: Rueterjans, H.]]
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[[Category: Rueterjans H]]
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[[Category: Veerkamp, J H.]]
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[[Category: Veerkamp JH]]
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[[Category: Zimmerman, A W.]]
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[[Category: Zimmerman AW]]
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[[Category: 15n isotope enrichment]]
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[[Category: beta barrel]]
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[[Category: fatty acid carrier]]
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[[Category: lipid binding protein]]
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[[Category: nmr spectroscopy]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:04:27 2008''
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Current revision

Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein

PDB ID 1jjx

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