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1nfa

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(New page: 200px<br /> <applet load="1nfa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nfa" /> '''HUMAN TRANSCRIPTION FACTOR NFATC DNA BINDIN...)
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[[Image:1nfa.gif|left|200px]]<br />
 
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'''HUMAN TRANSCRIPTION FACTOR NFATC DNA BINDING DOMAIN, NMR, 10 STRUCTURES'''<br />
 
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==Overview==
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==HUMAN TRANSCRIPTION FACTOR NFATC DNA BINDING DOMAIN, NMR, 10 STRUCTURES==
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Transcription factors of the NFAT family regulate the production of, effector proteins that coordinate the immune response. The, immunosuppressive drugs FK506 and cyclosporin A (CsA) act by blocking a, Ca2+-mediated signalling pathway leading to NFAT. Although FK506 and CsA, have enabled human organs to be transplanted routinely, the toxic, side-effects of these drugs limit their usage. This toxicity might be, absent in antagonists that target NFAT directly. As a first step in the, structure-based search for NFAT antagonists, we now report the, identification and solution structure of a 20K domain of NFATc (NFATc-DBD), that is both necessary and sufficient to bind DNA and activate, transcription cooperatively. Although the overall fold of the NFATc, DNA-binding domain is related to that of NF-kappaB p50 (refs 2, 3), the, two proteins use significantly different strategies for DNA recognition., On the basis of these results, we present a model for the cooperative, complex formed between NFAT and the mitogenic transcription factor AP-1 on, the interleukin-2 enhancer.
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<StructureSection load='1nfa' size='340' side='right'caption='[[1nfa]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nfa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NFA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nfa OCA], [https://pdbe.org/1nfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nfa RCSB], [https://www.ebi.ac.uk/pdbsum/1nfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nfa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NFAC1_HUMAN NFAC1_HUMAN] Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nf/1nfa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nfa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transcription factors of the NFAT family regulate the production of effector proteins that coordinate the immune response. The immunosuppressive drugs FK506 and cyclosporin A (CsA) act by blocking a Ca2+-mediated signalling pathway leading to NFAT. Although FK506 and CsA have enabled human organs to be transplanted routinely, the toxic side-effects of these drugs limit their usage. This toxicity might be absent in antagonists that target NFAT directly. As a first step in the structure-based search for NFAT antagonists, we now report the identification and solution structure of a 20K domain of NFATc (NFATc-DBD) that is both necessary and sufficient to bind DNA and activate transcription cooperatively. Although the overall fold of the NFATc DNA-binding domain is related to that of NF-kappaB p50 (refs 2, 3), the two proteins use significantly different strategies for DNA recognition. On the basis of these results, we present a model for the cooperative complex formed between NFAT and the mitogenic transcription factor AP-1 on the interleukin-2 enhancer.
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==About this Structure==
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Unusual Rel-like architecture in the DNA-binding domain of the transcription factor NFATc.,Wolfe SA, Zhou P, Dotsch V, Chen L, You A, Ho SN, Crabtree GR, Wagner G, Verdine GL Nature. 1997 Jan 9;385(6612):172-6. PMID:8990122<ref>PMID:8990122</ref>
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1NFA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NFA OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Unusual Rel-like architecture in the DNA-binding domain of the transcription factor NFATc., Wolfe SA, Zhou P, Dotsch V, Chen L, You A, Ho SN, Crabtree GR, Wagner G, Verdine GL, Nature. 1997 Jan 9;385(6612):172-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8990122 8990122]
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</div>
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<div class="pdbe-citations 1nfa" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Crabtree, G.R.]]
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[[Category: Crabtree GR]]
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[[Category: Dotsch, V.]]
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[[Category: Dotsch V]]
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[[Category: Ho, S.N.]]
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[[Category: Ho SN]]
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[[Category: Verdine, G.L.]]
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[[Category: Verdine GL]]
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[[Category: Wagner, G.]]
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[[Category: Wagner G]]
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[[Category: Wolfe, S.A.]]
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[[Category: Wolfe SA]]
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[[Category: You, A.]]
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[[Category: You A]]
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[[Category: Zhou, P.]]
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[[Category: Zhou P]]
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[[Category: activates cytokine transcription]]
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[[Category: nfat]]
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[[Category: rel-homology fold]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:21:00 2007''
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Current revision

HUMAN TRANSCRIPTION FACTOR NFATC DNA BINDING DOMAIN, NMR, 10 STRUCTURES

PDB ID 1nfa

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