1q9f

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(New page: 200px<br /><applet load="1q9f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q9f" /> '''NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN ...)
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[[Image:1q9f.gif|left|200px]]<br /><applet load="1q9f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q9f" />
 
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'''NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''<br />
 
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==Overview==
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==NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES==
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The structure of the integral membrane protein OmpX from Escherichia coli, reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526, NOE upper limit distance constraints. The structure determination was, based on complete sequence-specific assignments for the amide protons and, the Val, Leu, and Ile(delta1) methyl groups in OmpX, which were, selectively protonated on a perdeuterated background. The solution, structure of OmpX in the DHPC micelles consists of a well-defined, eight-stranded antiparallel beta-barrel, with successive pairs of, beta-strands connected by mobile loops. Several long-range NOEs observed, outside of the transmembrane barrel characterize an extension of a, four-stranded beta-sheet beyond the height of the barrel. This protruding, beta-sheet is believed to be involved in intermolecular interactions, responsible for the biological functions of OmpX. The present approach for, de novo structure determination should be quite widely applicable to, membrane proteins reconstituted in mixed micelles with overall molecular, masses up to about 100 kDa, and may also provide a platform for additional, functional studies.
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<StructureSection load='1q9f' size='340' side='right'caption='[[1q9f]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q9f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q9F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q9f OCA], [https://pdbe.org/1q9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q9f RCSB], [https://www.ebi.ac.uk/pdbsum/1q9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q9f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OMPX_ECOLI OMPX_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q9/1q9f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q9f ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val, Leu, and Ile(delta1) methyl groups in OmpX, which were selectively protonated on a perdeuterated background. The solution structure of OmpX in the DHPC micelles consists of a well-defined, eight-stranded antiparallel beta-barrel, with successive pairs of beta-strands connected by mobile loops. Several long-range NOEs observed outside of the transmembrane barrel characterize an extension of a four-stranded beta-sheet beyond the height of the barrel. This protruding beta-sheet is believed to be involved in intermolecular interactions responsible for the biological functions of OmpX. The present approach for de novo structure determination should be quite widely applicable to membrane proteins reconstituted in mixed micelles with overall molecular masses up to about 100 kDa, and may also provide a platform for additional functional studies.
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==About this Structure==
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NMR structure of the integral membrane protein OmpX.,Fernandez C, Hilty C, Wider G, Guntert P, Wuthrich K J Mol Biol. 2004 Mar 5;336(5):1211-21. PMID:15037080<ref>PMID:15037080</ref>
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1Q9F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q9F OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR structure of the integral membrane protein OmpX., Fernandez C, Hilty C, Wider G, Guntert P, Wuthrich K, J Mol Biol. 2004 Mar 5;336(5):1211-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15037080 15037080]
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</div>
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<div class="pdbe-citations 1q9f" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fernandez, C.]]
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[[Category: Fernandez C]]
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[[Category: Guntert, P.]]
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[[Category: Guntert P]]
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[[Category: Hilty, C.]]
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[[Category: Hilty C]]
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[[Category: Wider, G.]]
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[[Category: Wider G]]
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[[Category: Wuthrich, K.]]
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[[Category: Wuthrich K]]
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[[Category: detergents]]
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[[Category: dhpc]]
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[[Category: lipids]]
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[[Category: membrane protein]]
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[[Category: micelles]]
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[[Category: nmr]]
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[[Category: ompx]]
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[[Category: trosy]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:30:28 2007''
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NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES

PDB ID 1q9f

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