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| | ==Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination== | | ==Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination== |
| - | <StructureSection load='1sg5' size='340' side='right' caption='[[1sg5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1sg5' size='340' side='right'caption='[[1sg5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1sg5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SG5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1sg5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SG5 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YaeO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg5 OCA], [http://pdbe.org/1sg5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sg5 RCSB], [http://www.ebi.ac.uk/pdbsum/1sg5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sg5 ProSAT], [http://www.topsan.org/Proteins/BSGI/1sg5 TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg5 OCA], [https://pdbe.org/1sg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sg5 RCSB], [https://www.ebi.ac.uk/pdbsum/1sg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sg5 ProSAT], [https://www.topsan.org/Proteins/BSGI/1sg5 TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ROF_ECOLI ROF_ECOLI]] Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination. | + | [https://www.uniprot.org/uniprot/ROF_ECOLI ROF_ECOLI] Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Structural genomic]] | + | [[Category: Large Structures]] |
| - | [[Category: Gehring, K]] | + | [[Category: Gehring K]] |
| - | [[Category: Gutierrez, P]] | + | [[Category: Gutierrez P]] |
| - | [[Category: A+b protein]]
| + | |
| - | [[Category: Bsgi]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
ROF_ECOLI Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Rho-dependent transcription termination is an essential process for the regulation of bacterial gene expression. Thus far, only two Rho-specific inhibitors of bacterial transcription termination have been described, the psu protein from the satellite bacteriophage P4 and YaeO from Escherichia coli. Here, we report the solution structure of YaeO, the first of a Rho-specific inhibitor of transcription termination. YaeO is an acidic protein composed of an N-terminal helix and a seven-stranded beta sandwich. NMR chemical shift perturbation experiments revealed that YaeO binds proximal to the primary nucleic acid binding site of Rho. Based on the NMR titrations, a docked model of the YaeO-Rho complex was calculated. These results suggest that YaeO binds outside the Rho hexamer, acting as a competitive inhibitor of RNA binding. In vitro gel shift assays confirmed the inhibition of nucleic acid binding to Rho. Site-directed mutagenesis showed that the negative character of YaeO is essential for its function in vivo.
Solution structure of YaeO, a Rho-specific inhibitor of transcription termination.,Gutierrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K J Biol Chem. 2007 Aug 10;282(32):23348-53. Epub 2007 Jun 11. PMID:17565995[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gutierrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K. Solution structure of YaeO, a Rho-specific inhibitor of transcription termination. J Biol Chem. 2007 Aug 10;282(32):23348-53. Epub 2007 Jun 11. PMID:17565995 doi:10.1074/jbc.M702010200
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