2aqc

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(New page: 200px<br /><applet load="2aqc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aqc" /> '''NMR Structural analysis of archaeal Nop10'''...)
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[[Image:2aqc.gif|left|200px]]<br /><applet load="2aqc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2aqc" />
 
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'''NMR Structural analysis of archaeal Nop10'''<br />
 
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==Overview==
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==NMR Structural analysis of archaeal Nop10==
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Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA, pseudouridylation and direct processing of ribosomal RNA, and are, essential architectural components of vertebrate telomerases. H/ACA RNPs, comprise four proteins and a multihelical RNA. Two proteins, Cbf5 and, Nop10, suffice for basal enzymatic activity in an archaeal in vitro, system. We now report their cocrystal structure at 1.95-A resolution. We, find that archaeal Cbf5 can assemble with yeast Nop10 and with human, telomerase RNA, consistent with the high sequence identity of the RNP, components between archaea and eukarya. Thus, the Cbf5-Nop10 architecture, is phylogenetically conserved. The structure shows how Nop10 buttresses, the active site of Cbf5, and it reveals two basic troughs that, bidirectionally extend the active site cleft. Mutagenesis results, implicate an adjacent basic patch in RNA binding. This tripartite, RNA-binding surface may function as a molecular bracket that organizes the, multihelical H/ACA and telomerase RNAs.
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<StructureSection load='2aqc' size='340' side='right'caption='[[2aqc]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2aqc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AQC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aqc OCA], [https://pdbe.org/2aqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aqc RCSB], [https://www.ebi.ac.uk/pdbsum/2aqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aqc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOP10_METJA NOP10_METJA] Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aq/2aqc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aqc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA pseudouridylation and direct processing of ribosomal RNA, and are essential architectural components of vertebrate telomerases. H/ACA RNPs comprise four proteins and a multihelical RNA. Two proteins, Cbf5 and Nop10, suffice for basal enzymatic activity in an archaeal in vitro system. We now report their cocrystal structure at 1.95-A resolution. We find that archaeal Cbf5 can assemble with yeast Nop10 and with human telomerase RNA, consistent with the high sequence identity of the RNP components between archaea and eukarya. Thus, the Cbf5-Nop10 architecture is phylogenetically conserved. The structure shows how Nop10 buttresses the active site of Cbf5, and it reveals two basic troughs that bidirectionally extend the active site cleft. Mutagenesis results implicate an adjacent basic patch in RNA binding. This tripartite RNA-binding surface may function as a molecular bracket that organizes the multihelical H/ACA and telomerase RNAs.
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==About this Structure==
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The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs.,Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:16286935<ref>PMID:16286935</ref>
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2AQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AQC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs., Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR, Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16286935 16286935]
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</div>
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[[Category: Methanocaldococcus jannaschii]]
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<div class="pdbe-citations 2aqc" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Amare, A.R.Ferre-D.]]
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[[Category: Hamma, T.]]
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[[Category: Reichow, S.L.]]
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[[Category: Varani, G.]]
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[[Category: ZN]]
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[[Category: anop10]]
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[[Category: zinc-ribbon]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:17:44 2007''
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==See Also==
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*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Methanocaldococcus jannaschii]]
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[[Category: Ferre-D'Amare AR]]
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[[Category: Hamma T]]
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[[Category: Reichow SL]]
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[[Category: Varani G]]

Current revision

NMR Structural analysis of archaeal Nop10

PDB ID 2aqc

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