This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2k7w

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:35, 22 May 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2k7w.png|left|200px]]
 
-
<!--
+
==BAX Activation is Initiated at a Novel Interaction Site==
-
The line below this paragraph, containing "STRUCTURE_2k7w", creates the "Structure Box" on the page.
+
<StructureSection load='2k7w' size='340' side='right'caption='[[2k7w]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2k7w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K7W FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7w OCA], [https://pdbe.org/2k7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k7w RCSB], [https://www.ebi.ac.uk/pdbsum/2k7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7w ProSAT]</span></td></tr>
-
{{STRUCTURE_2k7w| PDB=2k7w | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/BAX_HUMAN BAX_HUMAN] Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.<ref>PMID:8358790</ref> <ref>PMID:10772918</ref> <ref>PMID:8521816</ref> <ref>PMID:16113678</ref> <ref>PMID:18948948</ref> <ref>PMID:21199865</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/2k7w_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k7w ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
BAX is a pro-apoptotic protein of the BCL-2 family that is stationed in the cytosol until activated by a diversity of stress stimuli to induce cell death. Anti-apoptotic proteins such as BCL-2 counteract BAX-mediated cell death. Although an interaction site that confers survival functionality has been defined for anti-apoptotic proteins, an activation site has not been identified for BAX, rendering its explicit trigger mechanism unknown. We previously developed stabilized alpha-helix of BCL-2 domains (SAHBs) that directly initiate BAX-mediated mitochondrial apoptosis. Here we demonstrate by NMR analysis that BIM SAHB binds BAX at an interaction site that is distinct from the canonical binding groove characterized for anti-apoptotic proteins. The specificity of the human BIM-SAHB-BAX interaction is highlighted by point mutagenesis that disrupts functional activity, confirming that BAX activation is initiated at this novel structural location. Thus, we have now defined a BAX interaction site for direct activation, establishing a new target for therapeutic modulation of apoptosis.
-
===BAX Activation is Initiated at a Novel Interaction Site===
+
BAX activation is initiated at a novel interaction site.,Gavathiotis E, Suzuki M, Davis ML, Pitter K, Bird GH, Katz SG, Tu HC, Kim H, Cheng EH, Tjandra N, Walensky LD Nature. 2008 Oct 23;455(7216):1076-81. PMID:18948948<ref>PMID:18948948</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2k7w" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_18948948}}, adds the Publication Abstract to the page
+
*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 18948948 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18948948}}
+
__TOC__
-
 
+
</StructureSection>
-
==Disease==
+
-
Known disease associated with this structure: Colorectal cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600040 600040]], T-cell acute lymphoblastic leukemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600040 600040]]
+
-
 
+
-
==About this Structure==
+
-
2K7W is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7W OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:18948948</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Bird, G H.]]
+
[[Category: Large Structures]]
-
[[Category: Cheng, E H.]]
+
[[Category: Bird GH]]
-
[[Category: Davis, M L.]]
+
[[Category: Cheng EH]]
-
[[Category: Gavathiotis, E.]]
+
[[Category: Davis ML]]
-
[[Category: Katz, S G.]]
+
[[Category: Gavathiotis E]]
-
[[Category: Kim, H.]]
+
[[Category: Katz SG]]
-
[[Category: Pitter, K.]]
+
[[Category: Kim H]]
-
[[Category: Suzuki, M.]]
+
[[Category: Pitter K]]
-
[[Category: Tjandra, N.]]
+
[[Category: Suzuki M]]
-
[[Category: Tu, H C.]]
+
[[Category: Tjandra N]]
-
[[Category: Walensky, L D.]]
+
[[Category: Tu HC]]
-
[[Category: Apoptosis]]
+
[[Category: Walensky LD]]
-
[[Category: Bax]]
+
-
[[Category: Bcl-2]]
+
-
[[Category: Bh3]]
+
-
[[Category: Bim]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 08:10:37 2009''
+

Current revision

BAX Activation is Initiated at a Novel Interaction Site

PDB ID 2k7w

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2k7w"
Personal tools