1s9h
From Proteopedia
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'''Crystal Structure of Adeno-associated virus Type 2 Rep40''' | '''Crystal Structure of Adeno-associated virus Type 2 Rep40''' | ||
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[[Category: Linden, R M.]] | [[Category: Linden, R M.]] | ||
[[Category: Yoon-Robarts, M.]] | [[Category: Yoon-Robarts, M.]] | ||
| - | [[Category: | + | [[Category: Aaa+ protein]] |
| - | [[Category: | + | [[Category: Helicase]] |
| - | [[Category: | + | [[Category: P-loop]] |
| - | [[Category: | + | [[Category: Sensor 1]] |
| - | [[Category: | + | [[Category: Walker some]] |
| - | [[Category: | + | [[Category: Walker b]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:27:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:27, 3 May 2008
Crystal Structure of Adeno-associated virus Type 2 Rep40
Overview
We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration.
About this Structure
1S9H is a Single protein structure of sequence from Adeno-associated virus 2. Full crystallographic information is available from OCA.
Reference
Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:12906833 Page seeded by OCA on Sat May 3 08:27:00 2008
