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2ccx

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DETERMINATION OF THE NUCLEAR MAGNETIC RESONANCE SOLUTION STRUCTURE OF CARDIOTOXIN CTX IIB FROM NAJA MOSSAMBICA MOSSAMBICA

Structural highlights

2ccx is a 1 chain structure with sequence from Naja mossambica. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

3SA1_NAJMO Shows cytolytic activity on many different cells by forming pore in lipid membranes. In vivo, increases heart rate or kills the animal by cardiac arrest. In addition, it binds to heparin with high affinity, interacts with Kv channel-interacting protein 1 (KCNIP1) in a calcium-independent manner, and binds to integrin alpha-V/beta-3 (ITGAV/ITGB3) with moderate affinity.[UniProtKB:P60301][UniProtKB:P60304]^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The NMR structure of cardiotoxin CTX IIb from Naja mossambica mossambica in aqueous solution was determined from a total of 593 nuclear Overhauser enhancement distance constraints and 135 dihedral angle constraints, which were collected using two-dimensional homonuclear 1H-NMR experiments. Structure calculations were performed with the program DIANA, using the redundant dihedral angle constraints strategy for improved convergence, followed by restrained energy minimization with the program FANTOM and a modified version of the program AMBER. The CTX IIb structure is represented by a group of 20 conformers with an average root-mean-square deviation relative to the mean solution structure of 0.072 nm for the backbone atoms, and 0.116 nm for all heavy atoms. The molecular structure of CTX IIb is characterized by a three-stranded beta-sheet made up of residues 20-26, 32-39 and 48-54, and a two-stranded beta-sheet composed of residues 1-5 and 10-14. A cluster of four disulfide bonds, 3-21, 14-38, 42-53 and 54-59, form the core of the molecule and crosslink the individual polypeptide strands. The NMR structure is similar to the previously reported X-ray crystal structure of the cardiotoxin CTX VII4 from the same species. Differences between the two structures were noted in the tips of the two loops formed by residues 6-9 and 27-31, which connect the beta-strand 1-5 with 10-14, and 20-26 with 32-39, respectively. For these loops the NMR data also indicate significantly increased dynamic disorder in the solution structure. These observations are discussed with respect to earlier suggestions by others that these two loops are essential structural elements for function and specificity of a wide variety of homologous toxins.

Determination of the nuclear-magnetic-resonance solution structure of cardiotoxin CTX IIb from Naja mossambica mossambica.,O'Connell JF, Bougis PE, Wuthrich K Eur J Biochem. 1993 May 1;213(3):891-900. PMID:8504828^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chien KY, Chiang CM, Hseu YC, Vyas AA, Rule GS, Wu W. Two distinct types of cardiotoxin as revealed by the structure and activity relationship of their interaction with zwitterionic phospholipid dispersions. J Biol Chem. 1994 May 20;269(20):14473-83. PMID:8182052
↑ O'Connell JF, Bougis PE, Wuthrich K. Determination of the nuclear-magnetic-resonance solution structure of cardiotoxin CTX IIb from Naja mossambica mossambica. Eur J Biochem. 1993 May 1;213(3):891-900. PMID:8504828

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ccx"

Categories: Large Structures | Naja mossambica | O'Connell J | Wuthrich K

@@ Line 1: / Line 1: @@
 ==DETERMINATION OF THE NUCLEAR MAGNETIC RESONANCE SOLUTION STRUCTURE OF CARDIOTOXIN CTX IIB FROM NAJA MOSSAMBICA MOSSAMBICA==
-<StructureSection load='2ccx' size='340' side='right'caption='[[2ccx]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2ccx' size='340' side='right'caption='[[2ccx]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ccx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mozambique_cobra Mozambique cobra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CCX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ccx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_mossambica Naja mossambica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CCX FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ccx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccx OCA], [http://pdbe.org/2ccx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ccx RCSB], [http://www.ebi.ac.uk/pdbsum/2ccx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ccx ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ccx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccx OCA], [https://pdbe.org/2ccx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ccx RCSB], [https://www.ebi.ac.uk/pdbsum/2ccx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ccx ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CX1_NAJMO CX1_NAJMO]] Shows cytolytic activity (By similarity).
+[https://www.uniprot.org/uniprot/3SA1_NAJMO 3SA1_NAJMO] Shows cytolytic activity on many different cells by forming pore in lipid membranes. In vivo, increases heart rate or kills the animal by cardiac arrest. In addition, it binds to heparin with high affinity, interacts with Kv channel-interacting protein 1 (KCNIP1) in a calcium-independent manner, and binds to integrin alpha-V/beta-3 (ITGAV/ITGB3) with moderate affinity.[UniProtKB:P60301][UniProtKB:P60304]<ref>PMID:8182052</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 36: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Mozambique cobra]]
+[[Category: Naja mossambica]]
-[[Category: Connell, J O]]
+[[Category: O'Connell J]]
-[[Category: Wuthrich, K]]
+[[Category: Wuthrich K]]
-[[Category: Cardiotoxin]]

2ccx

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Current revision

DETERMINATION OF THE NUCLEAR MAGNETIC RESONANCE SOLUTION STRUCTURE OF CARDIOTOXIN CTX IIB FROM NAJA MOSSAMBICA MOSSAMBICA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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