1sa3

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[[Image:1sa3.gif|left|200px]]
[[Image:1sa3.gif|left|200px]]
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{{Structure
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|PDB= 1sa3 |SIZE=350|CAPTION= <scene name='initialview01'>1sa3</scene>, resolution 1.95&Aring;
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The line below this paragraph, containing "STRUCTURE_1sa3", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= MSPIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=479 Moraxella sp.])
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|DOMAIN=
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{{STRUCTURE_1sa3| PDB=1sa3 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa3 OCA], [http://www.ebi.ac.uk/pdbsum/1sa3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sa3 RCSB]</span>
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'''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''
'''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''
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[[Category: Roberts, R J.]]
[[Category: Roberts, R J.]]
[[Category: Xu, Q S.]]
[[Category: Xu, Q S.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:28:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:18 2008''
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Revision as of 05:28, 3 May 2008

Image:1sa3.gif

Template:STRUCTURE 1sa3

An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site


Overview

Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.

About this Structure

1SA3 is a Single protein structure of sequence from Moraxella sp.. Full crystallographic information is available from OCA.

Reference

An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:15341737 Page seeded by OCA on Sat May 3 08:28:21 2008

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