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2cw9

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[[Image:2cw9.gif|left|200px]]
 
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{{Structure
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==Crystal structure of human Tim44 C-terminal domain==
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|PDB= 2cw9 |SIZE=350|CAPTION= <scene name='initialview01'>2cw9</scene>, resolution 1.90&Aring;
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<StructureSection load='2cw9' size='340' side='right'caption='[[2cw9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=1PE:PENTAETHYLENE GLYCOL'>1PE</scene>
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<table><tr><td colspan='2'>[[2cw9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CW9 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cw9 OCA], [https://pdbe.org/2cw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cw9 RCSB], [https://www.ebi.ac.uk/pdbsum/2cw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cw9 ProSAT], [https://www.topsan.org/Proteins/RSGI/2cw9 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TIM44_HUMAN TIM44_HUMAN] Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cw9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cw9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Familial oncocytic thyroid carcinoma is associated with a missense mutation, P308Q, in the C-terminal domain of Tim44. Tim44 is the mitochondrial inner-membrane translocase subunit and it functions as a membrane anchor for the mitochondrial heat-shock protein 70 (mtHsp70). Here, the crystal structure of the human Tim44 C-terminal domain complexed with pentaethylene glycol has been determined at 1.9 A resolution. The overall structure resembles that of the nuclear transport factor 2-like domain. In the crystal structure, pentaethylene glycol molecules are associated at two potential membrane-binding sites: the large hydrophobic cavity and the highly conserved loop between the alpha1 and alpha2 helices near Pro308. A comparison with the yeast homolog revealed that lipid binding induces conformational changes around the alpha1-alpha2 loop, leading to slippage of the alpha1 helix along the large beta-sheet. These changes may play important roles in the translocation of polypeptides across the mitochondrial inner membrane.
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'''Crystal structure of human Tim44 C-terminal domain'''
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Structure of the human Tim44 C-terminal domain in complex with pentaethylene glycol: ligand-bound form.,Handa N, Kishishita S, Morita S, Akasaka R, Jin Z, Chrzas J, Chen L, Liu ZJ, Wang BC, Sugano S, Tanaka A, Terada T, Shirouzu M, Yokoyama S Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1225-34. Epub 2007, Nov 16. PMID:18084070<ref>PMID:18084070</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2CW9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW9 OCA].
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<div class="pdbe-citations 2cw9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Chrzas, J.]]
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[[Category: Chrzas J]]
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[[Category: Handa, N.]]
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[[Category: Handa N]]
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[[Category: Jin, Z.]]
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[[Category: Jin Z]]
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[[Category: Kinoshita, Y.]]
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[[Category: Kinoshita Y]]
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[[Category: Kishishita, S.]]
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[[Category: Kishishita S]]
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[[Category: Liu, Z J.]]
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[[Category: Liu Z-J]]
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[[Category: Morita, S.]]
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[[Category: Morita S]]
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[[Category: Nagano, Y.]]
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[[Category: Nagano Y]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Shirouzu M]]
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[[Category: Shirouzu, M.]]
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[[Category: Takemoto C]]
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[[Category: Takemoto, C.]]
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[[Category: Terada T]]
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[[Category: Terada, T.]]
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[[Category: Uchikubo T]]
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[[Category: Uchikubo, T.]]
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[[Category: Uda H]]
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[[Category: Uda, H.]]
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[[Category: Wang B-C]]
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[[Category: Wang, B C.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: 1PE]]
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[[Category: national project on protein structural and functional analyse]]
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[[Category: nppfsa]]
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[[Category: nppsfa]]
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[[Category: protein transport]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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[[Category: structure genomic]]
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[[Category: tim]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:20:30 2008''
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Current revision

Crystal structure of human Tim44 C-terminal domain

PDB ID 2cw9

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