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2d51

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Pentaketide chromone synthase (M207G mutant)

Structural highlights

2d51 is a 2 chain structure with sequence from Aloe arborescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PCS_ALOAR Catalyzes the iterative condensations of 5 molecules of malonyl-CoA to produce a pentaketide 5,7-dihydroxy-2-methylchromone.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structures of a wild-type and a mutant PCS, a novel plant type III polyketide synthase from a medicinal plant, Aloe arborescens, were solved at 1.6 A resolution. The crystal structures revealed that the pentaketide-producing wild-type and the octaketide-producing M207G mutant shared almost the same overall folding, and that the large-to-small substitution dramatically increases the volume of the polyketide-elongation tunnel by opening a gate to two hidden pockets behind the active site of the enzyme. The chemically inert active site residue 207 thus controls the number of condensations of malonyl-CoA, solely depending on the steric bulk of the side chain. These findings not only provided insight into the polyketide formation reaction, but they also suggested strategies for the engineered biosynthesis of polyketides.

Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens.,Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T Chem Biol. 2007 Apr;14(4):359-69. PMID:17462571^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abe I, Utsumi Y, Oguro S, Morita H, Sano Y, Noguchi H. A plant type III polyketide synthase that produces pentaketide chromone. J Am Chem Soc. 2005 Feb 9;127(5):1362-3. PMID:15686354 doi:http://dx.doi.org/10.1021/ja0431206
↑ Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T. Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens. Chem Biol. 2007 Apr;14(4):359-69. PMID:17462571 doi:http://dx.doi.org/10.1016/j.chembiol.2007.02.003

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2d51"

Categories: Aloe arborescens | Large Structures | Kohno T | Morita H

@@ Line 1: / Line 1: @@
 ==Pentaketide chromone synthase (M207G mutant)==
-<StructureSection load='2d51' size='340' side='right' caption='[[2d51]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='2d51' size='340' side='right'caption='[[2d51]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2d51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aloar Aloar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D51 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2d51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aloe_arborescens Aloe arborescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D51 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2d3m|2d3m]], [[1bq6|1bq6]], [[1ee0|1ee0]], [[2d52|2d52]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d51 OCA], [http://pdbe.org/2d51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d51 RCSB], [http://www.ebi.ac.uk/pdbsum/2d51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d51 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d51 OCA], [https://pdbe.org/2d51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d51 RCSB], [https://www.ebi.ac.uk/pdbsum/2d51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d51 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PCS_ALOAR PCS_ALOAR]] Catalyzes the iterative condensations of 5 molecules of malonyl-CoA to produce a pentaketide 5,7-dihydroxy-2-methylchromone.<ref>PMID:15686354</ref>
+[https://www.uniprot.org/uniprot/PCS_ALOAR PCS_ALOAR] Catalyzes the iterative condensations of 5 molecules of malonyl-CoA to produce a pentaketide 5,7-dihydroxy-2-methylchromone.<ref>PMID:15686354</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Aloar]]
+[[Category: Aloe arborescens]]
-[[Category: Kohno, T]]
+[[Category: Large Structures]]
-[[Category: Morita, H]]
+[[Category: Kohno T]]
-[[Category: Chalcone synthase]]
+[[Category: Morita H]]
-[[Category: Pentaketide chromone synthase]]
-[[Category: Polyketide synthase]]
-[[Category: Transferase]]

2d51

From Proteopedia

Current revision

Pentaketide chromone synthase (M207G mutant)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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