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8wm4

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Current revision (18:08, 29 May 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8wm4 is ON HOLD until Paper Publication
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==Cryo-EM structure of DiCas7-11 in complex with crRNA==
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<StructureSection load='8wm4' size='340' side='right'caption='[[8wm4]], [[Resolution|resolution]] 2.93&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8wm4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfonema_ishimotonii Desulfonema ishimotonii] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WM4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.93&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wm4 OCA], [https://pdbe.org/8wm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wm4 RCSB], [https://www.ebi.ac.uk/pdbsum/8wm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wm4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A401FT36_9BACT A0A401FT36_9BACT]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CRISPR‒Cas7-11 is a Type III-E CRISPR-associated nuclease that functions as a potent RNA editing tool. Tetratrico-peptide repeat fused with Cas/HEF1-associated signal transducer (TPR-CHAT) acts as a regulatory protein that interacts with CRISPR RNA (crRNA)-bound Cas7-11 to form a CRISPR-guided caspase complex (Craspase). However, the precise modulation of Cas7-11's nuclease activity by TPR-CHAT to enhance its utility requires further study. Here, we report cryo-electron microscopy (cryo-EM) structures of Desulfonema ishimotonii (Di) Cas7-11-crRNA, complexed with or without the full length or the N-terminus of TPR-CHAT. These structures unveil the molecular features of the Craspase complex. Structural analysis, combined with in vitro nuclease assay and electrophoretic mobility shift assay, reveals that DiTPR-CHAT negatively regulates the activity of DiCas7-11 by preventing target RNA from binding through the N-terminal 65 amino acids of DiTPR-CHAT (DiTPR-CHAT(NTD)). Our work demonstrates that DiTPR-CHAT(NTD) can function as a small unit of DiCas7-11 regulator, potentially enabling safe applications to prevent overcutting and off-target effects of the CRISPR‒Cas7-11 system.
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Authors: Ma, H.Y., Tang, X.D.
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Structural basis of negative regulation of CRISPR-Cas7-11 by TPR-CHAT.,Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X Signal Transduct Target Ther. 2024 May 13;9(1):111. doi: , 10.1038/s41392-024-01821-4. PMID:38735995<ref>PMID:38735995</ref>
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Description: Cryo-EM structure of DiCas7-11 in complex with crRNA
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Ma, H.Y]]
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<div class="pdbe-citations 8wm4" style="background-color:#fffaf0;"></div>
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[[Category: Tang, X.D]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Desulfonema ishimotonii]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Ma HY]]
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[[Category: Tang XD]]

Current revision

Cryo-EM structure of DiCas7-11 in complex with crRNA

PDB ID 8wm4

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