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1pv6

From Proteopedia

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Current revision

Crystal structure of lactose permease

Structural highlights

1pv6 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACY_ECOLI Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane proteins. We report the crystal structure at 3.5 angstroms of the Escherichia coli lactose permease, an intensively studied member of the major facilitator superfamily of transporters. The molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease. A large internal hydrophilic cavity open to the cytoplasmic side represents the inward-facing conformation of the transporter. The structure with a bound lactose homolog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the cavity, and residues that play major roles in substrate recognition and proton translocation are identified. We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data.

Structure and mechanism of the lactose permease of Escherichia coli.,Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S Science. 2003 Aug 1;301(5633):610-5. PMID:12893935^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science. 2003 Aug 1;301(5633):610-5. PMID:12893935 doi:http://dx.doi.org/10.1126/science.1088196

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pv6"

@@ Line 3: / Line 3: @@
 <StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1pv7|1pv7]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [https://pdbe.org/1pv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
+[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Abramson, J]]
+[[Category: Abramson J]]
-[[Category: Iwata, S]]
+[[Category: Iwata S]]
-[[Category: Kaback, H R]]
+[[Category: Kaback HR]]
-[[Category: Kasho, V]]
+[[Category: Kasho V]]
-[[Category: Smirnova, I]]
+[[Category: Smirnova I]]
-[[Category: Verner, G]]
+[[Category: Verner G]]
-[[Category: Membrane protein]]
-[[Category: Sugar transport]]
-[[Category: Symport]]
-[[Category: Transport]]
-[[Category: Transport protein]]

1pv6

From Proteopedia

Current revision

Crystal structure of lactose permease

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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