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2e85

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Crystal Structure of the Hydrogenase 3 Maturation protease

Structural highlights

2e85 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

HYCI_ECOLI Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The maturation of [NiFe]-hydrogenases is a catalyzed process involving the activities of at least seven proteins. The last step consists of the endoproteolytic cleavage of the precursor of the large subunit, after the [NiFe]-metal center has been assembled. The HycI endopeptidase is involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3 from Escherichia coli. Although HycI has been well characterized biochemically, the crystallization of the protein has been quite challenging. Here, we present the crystal structure of HycI at 1.70 A resolution. The crystal structure resembles the recently reported solution structure (NMR) of the same protein and the holo-HyPD structure of the same family, but a significant conformational change is observed at the L5 loop, as compared with the solution structures of HycI and HyPD. In our crystal structure, three specific metal binding sites (Ca1-3) were identified and these metal ions are possibly involved in the C-terminal cleavage of HycE.

Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli.,Kumarevel T, Tanaka T, Bessho Y, Shinkai A, Yokoyama S Biochem Biophys Res Commun. 2009 Nov 13;389(2):310-4. Epub 2009 Aug 29. PMID:19720045^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumarevel T, Tanaka T, Bessho Y, Shinkai A, Yokoyama S. Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli. Biochem Biophys Res Commun. 2009 Nov 13;389(2):310-4. Epub 2009 Aug 29. PMID:19720045 doi:10.1016/j.bbrc.2009.08.135

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2e85"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Hydrogenase 3 Maturation protease==
-<StructureSection load='2e85' size='340' side='right' caption='[[2e85]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+<StructureSection load='2e85' size='340' side='right'caption='[[2e85]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2e85]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2E85 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2e85]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E85 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e85 OCA], [http://pdbe.org/2e85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2e85 RCSB], [http://www.ebi.ac.uk/pdbsum/2e85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2e85 ProSAT], [http://www.topsan.org/Proteins/RSGI/2e85 TOPSAN]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e85 OCA], [https://pdbe.org/2e85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e85 RCSB], [https://www.ebi.ac.uk/pdbsum/2e85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e85 ProSAT], [https://www.topsan.org/Proteins/RSGI/2e85 TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HYCI_ECOLI HYCI_ECOLI]] Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3.
+[https://www.uniprot.org/uniprot/HYCI_ECOLI HYCI_ECOLI] Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Kumarevel, T S]]
+[[Category: Large Structures]]
-[[Category: Structural genomic]]
+[[Category: Kumarevel TS]]
-[[Category: Shinkai, A]]
+[[Category: Shinkai A]]
-[[Category: Tanaka, T]]
+[[Category: Tanaka T]]
-[[Category: Yokoyama, S]]
+[[Category: Yokoyama S]]
-[[Category: Hydrogenase]]
-[[Category: Hydrolase]]
-[[Category: Maturation]]
-[[Category: National project on protein structural and functional analyse]]
-[[Category: Nppsfa]]
-[[Category: Protease]]
-[[Category: Rsgi]]

2e85

From Proteopedia

Current revision

Crystal Structure of the Hydrogenase 3 Maturation protease

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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