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2fh0

From Proteopedia

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[[Image:2fh0.jpg|left|200px]]<br /><applet load="2fh0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fh0" />
 
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'''NMR Ensemble of The Yeast Saccharomyces cerevisiae protein Ymr074cp core region'''<br />
 
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==Overview==
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==NMR Ensemble of The Yeast Saccharomyces cerevisiae protein Ymr074cp core region==
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A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics.
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<StructureSection load='2fh0' size='340' side='right'caption='[[2fh0]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fh0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FH0 FirstGlance]. <br>
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2FH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FH0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fh0 OCA], [https://pdbe.org/2fh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fh0 RCSB], [https://www.ebi.ac.uk/pdbsum/2fh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fh0 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural proteomics of an archaeon., Christendat D, Yee A, Dharamsi A, Kluger Y, Savchenko A, Cort JR, Booth V, Mackereth CD, Saridakis V, Ekiel I, Kozlov G, Maxwell KL, Wu N, McIntosh LP, Gehring K, Kennedy MA, Davidson AR, Pai EF, Gerstein M, Edwards AM, Arrowsmith CH, Nat Struct Biol. 2000 Oct;7(10):903-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11017201 11017201]
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== Function ==
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[https://www.uniprot.org/uniprot/YMW4_YEAST YMW4_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/2fh0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fh0 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Hong JJ]]
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[[Category: Hong, J J.]]
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[[Category: Shi YY]]
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[[Category: Shi, Y Y.]]
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[[Category: Wu JH]]
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[[Category: Wu, J H.]]
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[[Category: Zhang JH]]
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[[Category: Zhang, J H.]]
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[[Category: nmr ensemble]]
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[[Category: ymr074cp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:21:22 2008''
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Current revision

NMR Ensemble of The Yeast Saccharomyces cerevisiae protein Ymr074cp core region

PDB ID 2fh0

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