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| | ==NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain== | | ==NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain== |
| - | <StructureSection load='2fv4' size='340' side='right'caption='[[2fv4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2fv4' size='340' side='right'caption='[[2fv4]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2fv4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FV4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2fv4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FV4 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ftx|2ftx]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YER018C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), YMR117C, YM9718.16C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fv4 OCA], [https://pdbe.org/2fv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fv4 RCSB], [https://www.ebi.ac.uk/pdbsum/2fv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fv4 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fv4 OCA], [https://pdbe.org/2fv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fv4 RCSB], [https://www.ebi.ac.uk/pdbsum/2fv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fv4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> [[https://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref>
| + | [https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chou, J J]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Schnell, J R]] | + | [[Category: Chou JJ]] |
| - | [[Category: Alpha-beta]] | + | [[Category: Schnell JR]] |
| - | [[Category: Coiled-coil]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
SPC25_YEAST Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.
Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain.,Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC Structure. 2006 Jun;14(6):1003-9. PMID:16765893[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451
- ↑ McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:http://dx.doi.org/10.1101/gad.1040903
- ↑ Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC. Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain. Structure. 2006 Jun;14(6):1003-9. PMID:16765893 doi:10.1016/j.str.2006.04.007
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