2hde

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(New page: 200px<br /> <applet load="2hde" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hde" /> '''Solution Structure of Human SAP18'''<br /> ...)
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[[Image:2hde.gif|left|200px]]<br />
 
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<applet load="2hde" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hde" />
 
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'''Solution Structure of Human SAP18'''<br />
 
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==Overview==
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==Solution Structure of Human SAP18==
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Signal transduction pathways are frequently found to repress transcription, of target genes in the absence of stimulation and, conversely, to, upregulate transcription in the presence of a signal. Transcription, factors are central in this dual regulatory mechanism and widely use a, generalized mechanism to repress transcription through recruitment of a, Sin3-histone deacetylase (HDAC) complex to their binding sites on DNA. The, protein SAP18 (Sin3-associated polypeptide of 18 kDa) has been shown to, play a key role in gene-specific recruitment of the HDAC complex by a, number of transcription factors including Gli, GAGA, and Bicoid. The, solution structure of SAP18 reveals a ubiquitin-like fold with several, large loop insertions relative to other family members. This fold supports, the functional role of SAP18 as a protein-protein adapter module and, provides insight for how SAP18 may bridge the Sin3-HDAC complex to, transcription factors.
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<StructureSection load='2hde' size='340' side='right'caption='[[2hde]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hde]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HDE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HDE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hde FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hde OCA], [https://pdbe.org/2hde PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hde RCSB], [https://www.ebi.ac.uk/pdbsum/2hde PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hde ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SAP18_HUMAN SAP18_HUMAN] Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.<ref>PMID:9150135</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hd/2hde_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hde ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Signal transduction pathways are frequently found to repress transcription of target genes in the absence of stimulation and, conversely, to upregulate transcription in the presence of a signal. Transcription factors are central in this dual regulatory mechanism and widely use a generalized mechanism to repress transcription through recruitment of a Sin3-histone deacetylase (HDAC) complex to their binding sites on DNA. The protein SAP18 (Sin3-associated polypeptide of 18 kDa) has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. The solution structure of SAP18 reveals a ubiquitin-like fold with several large loop insertions relative to other family members. This fold supports the functional role of SAP18 as a protein-protein adapter module and provides insight for how SAP18 may bridge the Sin3-HDAC complex to transcription factors.
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==About this Structure==
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Structure of SAP18: a ubiquitin fold in histone deacetylase complex assembly.,McCallum SA, Bazan JF, Merchant M, Yin J, Pan B, de Sauvage FJ, Fairbrother WJ Biochemistry. 2006 Oct 3;45(39):11974-82. PMID:17002296<ref>PMID:17002296</ref>
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2HDE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HDE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of SAP18: a ubiquitin fold in histone deacetylase complex assembly., McCallum SA, Bazan JF, Merchant M, Yin J, Pan B, de Sauvage FJ, Fairbrother WJ, Biochemistry. 2006 Oct 3;45(39):11974-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17002296 17002296]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2hde" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Fairbrother, W.J.]]
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[[Category: McCallum, S.A.]]
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[[Category: Pan, B.]]
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[[Category: Yin, J.P.]]
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[[Category: bicoid]]
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[[Category: gaga]]
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[[Category: gli]]
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[[Category: hdac]]
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[[Category: histone deacetylase]]
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[[Category: sin3]]
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[[Category: transcriptional repressor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:30:42 2007''
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==See Also==
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*[[Histone deacetylase 3D structures|Histone deacetylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Fairbrother WJ]]
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[[Category: McCallum SA]]
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[[Category: Pan B]]
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[[Category: Yin JP]]

Current revision

Solution Structure of Human SAP18

PDB ID 2hde

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