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2jxm

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[[Image:2jxm.jpg|left|200px]]
 
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==Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex==
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The line below this paragraph, containing "STRUCTURE_2jxm", creates the "Structure Box" on the page.
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<StructureSection load='2jxm' size='340' side='right'caption='[[2jxm]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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{{STRUCTURE_2jxm| PDB=2jxm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxm OCA], [https://pdbe.org/2jxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxm RCSB], [https://www.ebi.ac.uk/pdbsum/2jxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS_PROHO PLAS_PROHO] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jx/2jxm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jxm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
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'''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex'''
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Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica.,Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089<ref>PMID:18201089</ref>
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==Overview==
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The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2JXM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA].
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</div>
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<div class="pdbe-citations 2jxm" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18201089 18201089]
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*[[Cytochrome f 3D structures|Cytochrome f 3D structures]]
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Prochlorothrix hollandica]]
[[Category: Prochlorothrix hollandica]]
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[[Category: Protein complex]]
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[[Category: Baranova M]]
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[[Category: Baranova, M.]]
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[[Category: Bullerjahn G]]
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[[Category: Bullerjahn, G.]]
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[[Category: Hulsker R]]
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[[Category: Hulsker, R.]]
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[[Category: Ubbink M]]
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[[Category: Ubbink, M.]]
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[[Category: Copper]]
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[[Category: Electron transport]]
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[[Category: Metal-binding]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:22:52 2008''
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Current revision

Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex

PDB ID 2jxm

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