1sj8
From Proteopedia
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'''Crystal Structure of talin residues 482-789''' | '''Crystal Structure of talin residues 482-789''' | ||
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[[Category: Gingras, A R.]] | [[Category: Gingras, A R.]] | ||
[[Category: Papagrigoriou, E.]] | [[Category: Papagrigoriou, E.]] | ||
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Revision as of 05:46, 3 May 2008
Crystal Structure of talin residues 482-789
Overview
The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions.
About this Structure
1SJ8 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle., Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J, EMBO J. 2004 Aug 4;23(15):2942-51. Epub 2004 Jul 22. PMID:15272303 Page seeded by OCA on Sat May 3 08:46:12 2008
