2rm8

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[[Image:2rm8.jpg|left|200px]]
 
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==The solution structure of phototactic transducer protein HtrII linker region from Natronomonas pharaonis==
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The line below this paragraph, containing "STRUCTURE_2rm8", creates the "Structure Box" on the page.
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<StructureSection load='2rm8' size='340' side='right'caption='[[2rm8]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2rm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RM8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rm8 OCA], [https://pdbe.org/2rm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rm8 RCSB], [https://www.ebi.ac.uk/pdbsum/2rm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rm8 ProSAT]</span></td></tr>
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{{STRUCTURE_2rm8| PDB=2rm8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HTR2_NATPH HTR2_NATPH] Transduces signals from the phototaxis receptor sensory rhodopsin II (SR-II) to the flagellar motor. Responds to light changes through the variation of the level of methylation. Also acts as a chemotransducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rm/2rm8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rm8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Halobacterial pharaonis phoborhodopsin [ppR, also called Natronomonas pharaonis sensory rhodopsin II (NpSRII)] is a phototaxis protein which transmits a light signal to the cytoplasm through its transducer protein (pHtrII). pHtrII, a two-transmembrane protein that interacts with ppR, belongs to the group of methyl-accepting chemotaxis proteins (MCPs). Several mutation studies have indicated that the linker region connecting the transmembrane and methylation regions is necessary for signal transduction. However, the three-dimensional (3D) structure of an MCP linker region has yet to be reported, and hence, details concerning the signal transduction mechanism remain unknown. Here the structure of the pHtrII linker region was investigated biochemically and biophysically. Following limited proteolysis, only one trypsin resistant fragment in the pHtrII linker region was identified. This fragment forms a homodimer with a Kd value of 115 muM. The 3D structure of this fragment was determined by solution NMR, and only one alpha-helix was found between two HAMP domains of the linker region. This alpha-helix was significantly stabilized within transmembrane protein pHtrII as revealed by CW-EPR. The presence of Af1503 HAMP domain-like structures in the linker region was supported by CD, NMR, and ELDOR data. The alpha-helix determined here presumably works as a mechanical joint between two HAMP domains in the linker region to transfer the photoactivated conformational change downstream.
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'''The solution structure of phototactic transducer protein HtrII linker region from Natronomonas pharaonis'''
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Structural Analysis of the Phototactic Transducer Protein HtrII Linker Region from Natronomonas pharaonis(,).,Hayashi K, Sudo Y, Jee J, Mishima M, Hara H, Kamo N, Kojima C Biochemistry. 2007 Dec 18;46(50):14380-14390. Epub 2007 Nov 15. PMID:18001143<ref>PMID:18001143</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==Overview==
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</div>
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Halobacterial pharaonis phoborhodopsin [ppR, also called Natronomonas pharaonis sensory rhodopsin II (NpSRII)] is a phototaxis protein which transmits a light signal to the cytoplasm through its transducer protein (pHtrII). pHtrII, a two-transmembrane protein that interacts with ppR, belongs to the group of methyl-accepting chemotaxis proteins (MCPs). Several mutation studies have indicated that the linker region connecting the transmembrane and methylation regions is necessary for signal transduction. However, the three-dimensional (3D) structure of an MCP linker region has yet to be reported, and hence, details concerning the signal transduction mechanism remain unknown. Here the structure of the pHtrII linker region was investigated biochemically and biophysically. Following limited proteolysis, only one trypsin resistant fragment in the pHtrII linker region was identified. This fragment forms a homodimer with a Kd value of 115 microM. The 3D structure of this fragment was determined by solution NMR, and only one alpha-helix was found between two HAMP domains of the linker region. This alpha-helix was significantly stabilized within transmembrane protein pHtrII as revealed by CW-EPR. The presence of Af1503 HAMP domain-like structures in the linker region was supported by CD, NMR, and ELDOR data. The alpha-helix determined here presumably works as a mechanical joint between two HAMP domains in the linker region to transfer the photoactivated conformational change downstream.
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<div class="pdbe-citations 2rm8" style="background-color:#fffaf0;"></div>
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== References ==
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==About this Structure==
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<references/>
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2RM8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RM8 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Structural analysis of the phototactic transducer protein HtrII linker region from Natronomonas pharaonis., Hayashi K, Sudo Y, Jee J, Mishima M, Hara H, Kamo N, Kojima C, Biochemistry. 2007 Dec 18;46(50):14380-90. Epub 2007 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18001143 18001143]
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[[Category: Natronomonas pharaonis]]
[[Category: Natronomonas pharaonis]]
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[[Category: Single protein]]
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[[Category: Hara H]]
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[[Category: Hara, H.]]
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[[Category: Hayashi K]]
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[[Category: Hayashi, K.]]
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[[Category: Jee J]]
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[[Category: Jee, J.]]
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[[Category: Kamo N]]
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[[Category: Kamo, N.]]
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[[Category: Kojima C]]
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[[Category: Kojima, C.]]
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[[Category: Mishima M]]
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[[Category: Mishima, M.]]
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[[Category: Sudo Y]]
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[[Category: Sudo, Y.]]
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[[Category: Chemotaxis]]
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[[Category: Chromophore]]
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[[Category: Membrane]]
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[[Category: Methylation]]
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[[Category: Photoreceptor protein]]
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[[Category: Protein]]
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[[Category: Receptor]]
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[[Category: Sensory transduction]]
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[[Category: Signaling protein]]
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[[Category: Transducer]]
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[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:09:36 2008''
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Current revision

The solution structure of phototactic transducer protein HtrII linker region from Natronomonas pharaonis

PDB ID 2rm8

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