3guz

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{{Seed}}
 
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[[Image:3guz.jpg|left|200px]]
 
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==Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's==
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The line below this paragraph, containing "STRUCTURE_3guz", creates the "Structure Box" on the page.
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<StructureSection load='3guz' size='340' side='right'caption='[[3guz]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
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{{STRUCTURE_3guz| PDB=3guz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [https://pdbe.org/3guz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [https://www.ebi.ac.uk/pdbsum/3guz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3guz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3guz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structural basis for the homotropic inhibition of pantothenate synthetase by the substrate pantoate was investigated by X-ray crystallography and high-resolution NMR spectroscopic methods. The tertiary structure of the dimeric N-terminal domain of Escherichia coli pantothenate synthetase, determined by X-ray crystallography to a resolution of 1.7 A, showed a second molecule of pantoate bound in the ATP-binding pocket. Pantoate binding to the ATP-binding site induced large changes in structure, mainly for backbone and side chain atoms of residues in the ATP binding HXGH(34-37) motif. Sequence-specific NMR resonance assignments and solution secondary structure of the dimeric N-terminal domain, obtained using samples enriched in (2)H, (13)C, and (15)N, indicated that the secondary structural elements were conserved in solution. Nitrogen-15 edited two-dimensional solution NMR chemical shift mapping experiments revealed that pantoate, at 10 mm, bound at these two independent sites. The solution NMR studies unambiguously demonstrated that ATP stoichiometrically displaced pantoate from the ATP-binding site. All NMR and X-ray studies were conducted at substrate concentrations used for enzymatic characterization of pantothenate synthetase from different sources [Jonczyk R &amp; Genschel U (2006) J Biol Chem 281, 37435-37446]. As pantoate binding to its canonical site is structurally conserved, these results demonstrate that the observed homotropic effects of pantoate on pantothenate biosynthesis are caused by competitive binding of this substrate to the ATP-binding site. The results presented here have implications for the design and development of potential antibacterial and herbicidal agents.
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===Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's===
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X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate.,Chakrabarti KS, Thakur KG, Gopal B, Sarma SP FEBS J. 2010 Feb;277(3):697-712. Epub 2010 Jan 4. PMID:20059543<ref>PMID:20059543</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3guz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20059543}}, adds the Publication Abstract to the page
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*[[Pantothenate synthetase|Pantothenate synthetase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20059543 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20059543}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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3GUZ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA].
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[[Category: Large Structures]]
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[[Category: Chakrabarti KS]]
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==Reference==
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[[Category: Gopal B]]
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<ref group="xtra">PMID:20059543</ref><references group="xtra"/>
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[[Category: Sarma SP]]
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[[Category: Escherichia coli]]
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[[Category: Thakur KG]]
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[[Category: Pantoate--beta-alanine ligase]]
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[[Category: Chakrabarti, K S.]]
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[[Category: Gopal, B.]]
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[[Category: Sarma, S P.]]
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[[Category: Thakur, K G.]]
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[[Category: Atp-binding]]
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[[Category: Competitive inhibition]]
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[[Category: Cytoplasm]]
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[[Category: Ligase]]
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[[Category: Non-canonical pantoate binding-site]]
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[[Category: Nucleotide-binding]]
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[[Category: Pantothenate biosynthesis]]
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[[Category: Rossmann fold]]
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[[Category: Substrate binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 10 17:22:51 2010''
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Current revision

Structural and substrate-binding studies of pantothenate synthenate (PS)provide insights into homotropic inhibition by pantoate in PS's

PDB ID 3guz

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