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4ou6

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Crystal structure of DnaT84-153-dT10 ssDNA complex form 1

Structural highlights

4ou6 is a 6 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.96Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNAT_ECOLI This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N a prepriming protein complex on the specific site of the template DNA recognized by protein N'.[HAMAP-Rule:MF_01061]

Publication Abstract from PubMed

DnaT is a primosomal protein that is required for the stalled replication fork restart in Escherichia coli. As an adapter, DnaT mediates the PriA-PriB-ssDNA ternary complex and the DnaB/C complex. However, the fundamental function of DnaT during PriA-dependent primosome assembly is still a black box. Here, we report the 2.83 A DnaT84-153-dT10 ssDNA complex structure, which reveals a novel three-helix bundle single-stranded DNA binding mode. Based on binding assays and negative-staining electron microscopy results, we found that DnaT can bind to phiX 174 ssDNA to form nucleoprotein filaments for the first time, which indicates that DnaT might function as a scaffold protein during the PriA-dependent primosome assembly. In combination with biochemical analysis, we propose a cooperative mechanism for the binding of DnaT to ssDNA and a possible model for the assembly of PriA-PriB-ssDNA-DnaT complex that sheds light on the function of DnaT during the primosome assembly and stalled replication fork restart. This report presents the first structure of the DnaT C-terminal complex with ssDNA and a novel model that explains the interactions between the three-helix bundle and ssDNA.

Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.,Liu Z, Chen P, Wang X, Cai G, Niu L, Teng M, Li X Nucleic Acids Res. 2014 Jul 22. pii: gku633. PMID:25053836^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu Z, Chen P, Wang X, Cai G, Niu L, Teng M, Li X. Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode. Nucleic Acids Res. 2014 Jul 22. pii: gku633. PMID:25053836 doi:http://dx.doi.org/10.1093/nar/gku633

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ou6"

@@ Line 1: / Line 1: @@
 ==Crystal structure of DnaT84-153-dT10 ssDNA complex form 1==
-<StructureSection load='4ou6' size='340' side='right' caption='[[4ou6]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
+<StructureSection load='4ou6' size='340' side='right'caption='[[4ou6]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ou6]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ou6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OU6 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ou7|4ou7]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou6 OCA], [http://pdbe.org/4ou6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ou6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou6 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ou6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou6 OCA], [https://pdbe.org/4ou6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ou6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ou6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou6 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DNAT_ECOLI DNAT_ECOLI]] This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.[HAMAP-Rule:MF_01061]
+[https://www.uniprot.org/uniprot/DNAT_ECOLI DNAT_ECOLI] This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.[HAMAP-Rule:MF_01061]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Chen, P]]
+[[Category: Escherichia coli K-12]]
-[[Category: Li, X]]
+[[Category: Large Structures]]
-[[Category: Liu, Z]]
+[[Category: Chen P]]
-[[Category: Niu, L]]
+[[Category: Li X]]
-[[Category: Teng, M]]
+[[Category: Liu Z]]
-[[Category: Dna binding]]
+[[Category: Niu L]]
-[[Category: Replication-dna complex]]
+[[Category: Teng M]]

4ou6

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Current revision

Crystal structure of DnaT84-153-dT10 ssDNA complex form 1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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