7k8c
From Proteopedia
(Difference between revisions)
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==CryoEM structure of a trehalose monomycolate transporter in lipid nanodiscs== | ==CryoEM structure of a trehalose monomycolate transporter in lipid nanodiscs== | ||
| - | <StructureSection load='7k8c' size='340' side='right'caption='[[7k8c]]' scene=''> | + | <StructureSection load='7k8c' size='340' side='right'caption='[[7k8c]], [[Resolution|resolution]] 4.27Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K8C FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K8C FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k8c OCA], [https://pdbe.org/7k8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k8c RCSB], [https://www.ebi.ac.uk/pdbsum/7k8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k8c ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.27Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k8c OCA], [https://pdbe.org/7k8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k8c RCSB], [https://www.ebi.ac.uk/pdbsum/7k8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k8c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The mycobacterial membrane protein large 3 (MmpL3) transporter is essential and required for shuttling the lipid trehalose monomycolate (TMM), a precursor of mycolic acid (MA)-containing trehalose dimycolate (TDM) and mycolyl arabinogalactan peptidoglycan (mAGP), in Mycobacterium species, including Mycobacterium tuberculosis and Mycobacterium smegmatis. However, the mechanism that MmpL3 uses to facilitate the transport of fatty acids and lipidic elements to the mycobacterial cell wall remains elusive. Here, we report 7 structures of the M. smegmatis MmpL3 transporter in its unbound state and in complex with trehalose 6-decanoate (T6D) or TMM using single-particle cryo-electron microscopy (cryo-EM) and X-ray crystallography. Combined with calculated results from molecular dynamics (MD) and target MD simulations, we reveal a lipid transport mechanism that involves a coupled movement of the periplasmic domain and transmembrane helices of the MmpL3 transporter that facilitates the shuttling of lipids to the mycobacterial cell wall. | ||
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| + | Structures of the mycobacterial membrane protein MmpL3 reveal its mechanism of lipid transport.,Su CC, Klenotic PA, Cui M, Lyu M, Morgan CE, Yu EW PLoS Biol. 2021 Aug 12;19(8):e3001370. doi: 10.1371/journal.pbio.3001370., eCollection 2021 Aug. PMID:34383749<ref>PMID:34383749</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7k8c" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Su C-C]] | [[Category: Su C-C]] | ||
Current revision
CryoEM structure of a trehalose monomycolate transporter in lipid nanodiscs
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