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7v9e
From Proteopedia
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<StructureSection load='7v9e' size='340' side='right'caption='[[7v9e]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='7v9e' size='340' side='right'caption='[[7v9e]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V9E FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=GUN:GUANINE'>GUN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v9e OCA], [https://pdbe.org/7v9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v9e RCSB], [https://www.ebi.ac.uk/pdbsum/7v9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v9e ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v9e OCA], [https://pdbe.org/7v9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v9e RCSB], [https://www.ebi.ac.uk/pdbsum/7v9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v9e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but in vitro selection has generated species that catalyze a broader range of chemistry; yet, there have been few structural and mechanistic studies of selected ribozymes. A ribozyme has recently been selected that can catalyze a site-specific methyl transfer reaction. We have solved the crystal structure of this ribozyme at a resolution of 2.3 A, showing how the RNA folds to generate a very specific binding site for the methyl donor substrate. The structure immediately suggests a catalytic mechanism involving a combination of proximity and orientation and nucleobase-mediated general acid catalysis. The mechanism is supported by the pH dependence of the rate of catalysis. A selected methyltransferase ribozyme can thus use a relatively sophisticated catalytic mechanism, broadening the range of known RNA-catalyzed chemistry. | ||
| - | + | ==See Also== | |
| - | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Deng | + | [[Category: Deng J]] |
| - | [[Category: Huang | + | [[Category: Huang L]] |
| - | [[Category: Lilley | + | [[Category: Lilley DMJ]] |
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Current revision
Crystal structure of a methyl transferase ribozyme
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