This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


Beta-lactamase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:15, 30 May 2024) (edit) (undo)
 
(49 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3bm6.png|left|200px|thumb|Crystal Structure of Beta-lactamase, [[3bm6]]]]
+
<StructureSection load='' size='350' side='right' scene='45/453531/Cv/1' caption='Class B β-lactamase Ndm-1 complex with Zn+2 (grey) (PDB code [[3spu]])'>
-
{{STRUCTURE_3bm6| PDB=3bm6 | SIZE=400| SCENE=Beta-lactamase/Beta-lactamase/1|right|CAPTION=Beta-lactamase class D dimer complex with boronic acid derivative, [[3bm6]] }}
+
__TOC__
-
 
+
== Function ==
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
'''Beta-lactamase''' (Blac) are enzymes which make bacteria resistant to β-lactam antibiotics by breaking their β-lactam ring. Blac are classified according to their resistance targets. Class C are cephalosporinases; Class A are broad-spectrum; Class B are metalloenzymes; Class D are cloxacilanases.
+
-
 
+
-
 
+
-
 
+
-
{{TOC limit|limit=2}}
+
-
 
+
-
 
+
 +
'''Beta-lactamase''' (Blac) are enzymes which make bacteria resistant to β-lactam antibiotics by breaking their β-lactam ring. Blac are classified according to their resistance targets.<br />
 +
*'''Class A''' are broad-spectrum. See also [[TEM1 Class Antibiotic Resistance Proteins]].<br />
 +
*'''Class B''' are metalloenzymes. See also [[New Delhi metallo-β-lactamase 1]].<br />
 +
*'''Class C''' are cephalosporinases. See also [[Class C beta-lactamase]].<br />
 +
*'''Class D''' are cloxacilanases<br />
 +
*'''Carbapenemase''' (Car) are Blac with wide hydrolytic capacities. Car can hydrolyze penicillins, cephalosporins, monobactams and carbapenems. <br />
 +
*'''Extended-spectrum Blac''' class A (ESBL) hydrolyze penicillins but not cephalosporins.<br />
 +
*'''Serine beta-lactamase''' (ClbP) has a serine-active site.
 +
*'''AmpC beta-lactamase''' (ClbP) mediate resistance to cephalosphin, cefazolin,cefoxitin and most penicillins<ref>PMID:19136439</ref>.
 +
== Relevance ==
 +
Blac makes some bacteria resistant to antibiotics like penicillin, cephamycin and carbapenems.
 +
== Structural highlights ==
 +
The <scene name='45/453531/Cv/3'>active site of class B Blac contains 2 Zn+2 ions</scene>.<ref>PMID:21774017</ref>
 +
==See also==
 +
[[Journal:Structure:1]]
== 3D Structures of Beta-lactamase ==
== 3D Structures of Beta-lactamase ==
 +
[[Beta-lactamase 3D structures]]
-
''Update June 2012''
+
</StructureSection>
-
 
+
-
===β-lactamase class A===
+
-
 
+
-
[[3dtm]], [[1bza]], [[1ck3]], [[1esu]], [[1jwp]], [[1lhy]], [[1li0]], [[1m40]], [[1zg6]], [[2v1z]], [[2v20]], [[3cmz]], [[3jyi]] - EcBlac A Tem-1 (mutant) - ''Escherichia coli''<br />
+
-
[[1btl]], [[1iys]], [[1xpb]], [[1zg4]] - EcBlac A Tem-1<br />
+
-
[[1lhy]] - EcBlac A Tem-30 (mutant) <br />
+
-
[[1li0]] - EcBlac A Tem-32 (mutant) <br />
+
-
[[1li9]] - EcBlac A Tem-34 (mutant) <br />
+
-
[[1yt4]] - EcBlac A Tem-76 (mutant) <br />
+
-
[[3toi]] - EcBlac A Tem-116 (mutant)<br />
+
-
[[1we4]], [[2xr0]], [[2zq7]], [[2zq8]], [[2zq9]], [[2zqa]], [[2zqc]], [[2zqd]] - EcBlac A Toho-1 (mutant) <br />
+
-
[[2xqz]], [[2wyx]] - EcBlac A Toho-1 (mutant) – Neutron<br />
+
-
[[3c5a]] – EcBlac A Kpc-2<br />
+
-
[[1ylj]], [[1ylp]], [[1ylt]], [[1ylw]], [[1yly]], [[1ylz]], [[1ym1]], [[1yms]], [[1ymx]], [[2p74]] – EcBlac A Ctx-M<br />
+
-
[[3hre]] - EcBlac A Ctx-M (mutant) <br />
+
-
[[1o7e]], [[1n4o]] – StmBlac A – ''Stenotrophonas maltophilia''<br />
+
-
[[1htz]] – KpBlac A Tem-52 – ''Klebsiella pneumoniae''<br />
+
-
[[1shv]] - KpBlac A Shv-1<br />
+
-
[[1tdl]], [[2a3u]], [[3oph]], [[3opl]] - KpBlac A Shv-1 (mutant) <br />
+
-
[[1n9b]] – KpBlac A Shv-2<br />
+
-
[[2qpn]] – KpBlac A Ges-1<br />
+
-
[[3ni9]] - PaBlac A Ges-2<br />
+
-
[[1hzo]] – Blac A – ''Proteus vulgaris''<br />
+
-
[[1kge]], [[1kgf]], [[1kgg]], [[1ome]]– SaBlac A (mutant) - S''taphylococcus aureus''<br />
+
-
[[3blm]] – SaBlac A<br />
+
-
[[1mbl]], [[3ly3]], [[3soi]] – BlBlac A (mutant) – ''Bacillus licheniformis''<br />
+
-
[[2blm]], [[3sh7]] – BlBlac A<br />
+
-
[[2cc1]] – Blac A – ''Mycobacterium fortuitum''<br />
+
-
[[3bfd]], [[3bfe]] – CsBlac A Sed-1 (mutant) - ''Citrobacter sedlakii''
+
-
 
+
-
===β-lactamase class A binary complex===
+
-
 
+
-
[[1axb]] - EcBlac A (mutant) + transition state analog<br />
+
-
[[1tem]], [[1bt5]], [[1fqg]], [[1iyo]], [[1iyp]], [[1iyq]], [[1jvj]], [[3q07]], [[3q1f]] - EcBlac A (mutant) + antibiotic<br />
+
-
[[1nxy]], [[1ny0]], [[1nym]], [[1nyy]] - EcBlac A (mutant) + boronic acid inhibitor<br />
+
-
[[1pzo]], [[1pzp]] - EcBlac A Tem-1 (mutant) + inhibitor<br />
+
-
[[1erm]], [[1ero]], [[1erq]] – EcBlac A Tem-1 + boronic acid inhibitor<br />
+
-
[[3g2y]], [[3g2z]], [[3g30]], [[3g31]], [[3g32]], [[3g34]], [[3g35]], [[4dds]], [[4ddy]], [[4de0]], [[4de1]], [[4de2]], [[4de3]] - EcBlac A Ctx-M + inhibitor<br />
+
-
[[3hlw]], [[3huo]], [[3hvf]] - EcBlac A Ctx-M (mutant) + antibiotic<br />
+
-
[[1jwv]], [[1jwz]] - EcBlac A Tem-1 (mutant) + boronic acid inhibitor<br />
+
-
[[1ong]], [[1q2p]], [[1vm1]], [[2h5s]], [[2zd8]], [[3d4f]], [[3mke]], [[3mkf]], [[3mxr]], [[3mxs]] - KpBlac A Shv-1 + inhibitor<br />
+
-
[[1rcj]], [[1tdg]], [[2a49]], [[2h0t]], [[2h0y]], [[2h10]] - KpBlac A Shv-1 (mutant) + inhibitor<br />
+
-
[[3opp]], [[3opr]]- KpBlac A Shv-1 (mutant) + antibiotic<br />
+
-
[[1w7f]], [[3b3x]], [[2y91]] - BlBlac A + inhibitor<br />
+
-
[[3m2j]] - BlBlac A (mutant) + inhibitor<br />
+
-
[[2wk0]], [[2x71]]– BlBlac A + antibiotic<br />
+
-
[[3ly4]], [[3m2k]], [[3sh8]], [[3sh9]] - BlBlac A (mutant) + antibiotic<br />
+
-
[[2q9m]], [[2q9n]] - EncBlac A + inhibitor – ''Enterobacter cloacae''<br />
+
-
[[3bfc]], [[3bff]], [[3bfg]] – CsBlac A Sed-1 (mutant) + inhibitor<br />
+
-
[[3nia]] - PaBlac A Ges-2 + inhibitor<br />
+
-
[[3p98]] – Blac Tem-72 + citrate – ''Morganella morganii''
+
-
 
+
-
===β-lactamase class A complex with β-lactamase inhibitor protein (Blip)===
+
-
 
+
-
[[3c7u]], [[3c7v]], [[1jtg]], [[2b5r]] – EcBlac A (mutant) + Protein Blip<br />
+
-
[[1jtd]], [[3gmw]] - EcBlac A + Protein Blip<br />
+
-
[[1s0w]], [[1xxm]] - EcBlac A + Protein Blip (mutant) <br />
+
-
[[2g2u]] - KpBlac A Shv-1 + Protein Blip<br />
+
-
[[2g2w]], [[3n4i]] - KpBlac A Shv-1 (mutant) + Protein Blip<br />
+
-
[[3c4o]], [[3c4p]] - KpBlac A Shv-1 + Protein Blip (mutant) <br />
+
-
[[3e2k]], [[3e2l]] - KpBlac A Kpc-2 (mutant) + Protein Blip<br />
+
-
[[3qhv]], [[2qhy]] - Blac A + Protein Blip – ''Bacillus anthracis''
+
-
 
+
-
 
+
-
===β-lactamase class B (Zn containing)===
+
-
 
+
-
[[3fcz]], [[3i0v]], [[3i11]], [[3i13]], [[3i14]], [[3i15]], [[1bc2]], [[1bvt]], [[1mqo]], [[2bc2]], [[3bc2]], [[1bmc]] – BcBlac B - ''Bacillus cereus''<br />
+
-
[[3knr]], [[3kns]], [[1dxk]], [[2bfk]], [[2bfl]], [[2bfz]], [[2bg2]], [[2bg6]], [[2bg7]], [[2bg8]], [[2bga]], [[2nxa]], [[2nyp]], [[2nze]], [[2nzf]], [[2uyx]] - BcBlac B (mutant) <br />
+
-
[[1a7t]], [[1znb]], [[2bmi]] – BfBlac B – ''Bacterioides fragilis''<br />
+
-
[[2znb]] - BfBlac B (Cd) <br />
+
-
[[3znb]] – BfBlac B (Zn, Hg) <br />
+
-
[[4znb]] - BfBlac B (mutant) <br />
+
-
[[1k07]] – FgBlac B]] - ''Fluoribacter gormanii''<br />
+
-
[[1l9y]] - FgBlac B (mutant) <br />
+
-
[[1ko2]], [[1ko3]] – PaBlac B Vim-2 - ''Pseudomonas aeruginosa''<br />
+
-
[[2whg]], [[2wrs]] - PaBlac B Vim-4<br />
+
-
[[2y87]], [[2y8a]], [[2y8b]] - PaBlac B Vim-7<br />
+
-
[[2fhx]] - PaBlac B Spm-1<br />
+
-
[[2x01]] - PaBlac Oxa-10 (mutant)<br />
+
-
[[2x02]] - PaBlac Oxa-10<br />
+
-
[[1wuo]], [[1wup]] - SmBlac Imp-1 (mutant) – ''Serratia marcescens''<br />
+
-
[[2dkf]], [[2zo4]], [[2zwr]] – TtBlac B – ''Thermus thermophilus''<br />
+
-
[[2zzi]] – TtBlac B (Fe) <br />
+
-
[[2fm6]], [[2fu6]], [[2fu7]], [[2h6a]] – StmMlac B<br />
+
-
[[2qin]], [[2qjs]] - StmBlac B (mutant) <br />
+
-
[[2gmn]], [[3lvz]] – BjBlac B – ''Bradyrhizobium japonicum''<br />
+
-
[[2p4z]] – Blac B – ''Thermoanaerobacter tengcongensis''<br />
+
-
[[3f9o]] - AhBlac B – ''Aeromonas hydrophila''<br />
+
-
[[3fai]], [[1x8h]] - AhBlac B (mutant) <br />
+
-
[[3l6n]] – Blac B – ''Chryseobacter indolegenes''<br />
+
-
[[3r2u]] – SaBlac B<br />
+
-
[[3rkj]], [[3sbl]], [[3sfp]], [[3spu]], [[3zr9]] - KpBlac B Ndm-1<br />
+
-
[[3srx]] - KpBlac B Ndm-1 (Cd) <br />
+
-
[[3rkk]] - KpBlac B Ndm-1 (mutant) <br />
+
-
[[3s0z]] - EcBlac B Ndm-1<br />
+
-
[[2yz3]] – Blac Vim-2 – ''Pseudomonas putida''<br />
+
-
[[3sd9]] – SfBlac B - ''Serratia fonticola''
+
-
 
+
-
===β-lactamase class B binary complex===
+
-
 
+
-
[[1a8t]], [[1hlk]], [[1kr3]] - BfBlac B + inhibitor<br />
+
-
[[1jt1]] – FgBlac + captopril <br />
+
-
[[1jje]], [[1jjt]], [[3lce]] - PaBlac B + inhibitor<br />
+
-
[[1m2x]] – Blac B + captopril – ''Elizabethkingia meningoseptica''<br />
+
-
[[1vgn]] - SmBlac Imp-1+ inhibitor<br />
+
-
[[2doo]] - SmBlac Imp-1+ fluorescent agent<br />
+
-
[[2aio]] – StmBlac B + antibiotic<br />
+
-
[[2fu8]], [[2fu9]], [[2gfj]], [[2gfk]], [[2hb9]], [[2qdt]] - StmBlac B + inhibitor<br />
+
-
[[3iof]], [[1x8i]], [[3iog]]– AhBlac B (mutant) + inhibitor<br />
+
-
[[1x8g]], [[2gkl]] – AhBlac B + inhibitor<br />
+
-
[[2qds]] – AhBlac B + captopril<br />
+
-
[[3m8t]] – BjBlac B + inhibitor<br />
+
-
[[3q6v]] – SfBlac B + glycerol <br />
+
-
[[3q6x]] – KpBlac B Ndm-1+ antibiotic<br />
+
-
 
+
-
===β-lactamase class C===
+
-
 
+
-
[[3fkw]], [[3iwi]], [[3iwo]], [[3iwq]], [[3ixd]] – EcBlac C (mutant) <br />
+
-
[[1zkj]] – Blac C – ''Enterobacter aerogenes''<br />
+
-
[[2qz6]] – Blac C – ''Pseudomonas fluorescens''
+
-
 
+
-
===β-lactamase class C binary complex===
+
-
 
+
-
[[3bm6]], [[3fkv]], [[3o86]], [[3o87]], [[3o88]] – EcBlac C + boronic acid inhibitor<br />
+
-
[[3ixb]], [[3ixg]] - EcBlac C (mutant) + boronic acid inhibitor<br />
+
-
[[3ixh]] - EcBlac C (mutant) + cefotaxime<br />
+
-
[[3gqz]], [[3gr2]], [[3grj]], [[3gsg]], [[3gtc]], [[3gv9]], [[3gvb]] - EcBlac C + inhibitor<br />
+
-
[[1onh]], [[1q2q]], [[1y54]] - EncBlac C + inhibitor<br />
+
-
[[1s6r]] - EncBlac C+ boronic acid inhibitor<br />
+
-
[[1rgz]] - EncBlac C + antribiotic<br />
+
-
[[1rgy]] - CfBlac C + antribiotic – ''Citrobacter freundii''<br />
+
-
[[1zc2]], [[2zc7]] – KpBlac C + inhibitor<br />
+
-
[[2wzx]], [[2wzz]] – PaBlac C + inhibitor<br />
+
-
 
+
-
===β-lactamase class D===
+
-
 
+
-
[[2rl3]], [[1k55]], [[1k57]], [[1k4e]], [[1ewz]], [[1fof]], [[1ddk]], [[1e3u]], [[1e4d]], [[1h8z]] – PaBlac D <br />
+
-
[[2hp5]], [[2hp6]], [[2hp9]], [[2hpb]], [[2wgw]], [[2wki]] - PaBlac D (mutant) <br />
+
-
[[1k56]], [[2k02]] - PaBlac Pse-2<br />
+
-
[[1g68]], [[1k4f]] – PaBlac Pse-4<br />
+
-
[[1g6a]] – PaBlac Pse-4 (mutant) <br />
+
-
[[1ko2]] – PaBlac Vim-2<br />
+
-
[[1k38]] – Blac D – ''Salmonella typhimurium''<br />
+
-
[[1m6k]] – EcBlac D<br />
+
-
[[3hbr]] – KpBlac D Oxa-48<br />
+
-
[[3if6]] - KpBlac D Oxa-46
+
-
 
+
-
===β-lactamase class D binary complex===
+
-
 
+
-
[[1k6r]], [[1k54]] - PaBlac D + antibiotic<br />
+
-
[[2wgi]], [[2wkh]] - PaBlac D (mutant) + antibiotic<br />
+
-
[[1k6s]] - PaBlac D + phenylboronic acid<br />
+
-
[[1dd6]], [[1h5x]], [[1h8y]], [[2wgv]] - PaBlac D + inhibitor<br />
+
-
[[3isg]] – EcBlac D + inhibitor<br />
+
-
[[3pae]], [[3pag]] - AbBlac D (mutant) + inhibitor<br />
+
-
 
+
-
===AmpC β-lactamase===
+
-
 
+
-
[[1ke4]], [[2bls]], [[2zj9]], [[3bls]] – EcBlac<br />
+
-
[[1l0d]], [[1l0e]], [[1l0f]], [[1l0g]] - EcBlac (mutant)
+
-
[[1fcn ]]– EcBlac (mutant) + substrate<br />
+
-
[[1fco]], [[1i5q]], [[1kvl]] - EcBlac (mutant) + antibiotic<br />
+
-
[[1iel]], [[1fcm]], [[1iem]], [[1kvm]], [[1ll5]], [[1ll9]], [[1llb]] - EcBlac + antibiotic<br />
+
-
[[1fsw]], [[1fsy]], [[1kds]], [[1kdw]], [[1ke0]], [[1ke3]], [[1mxo]], [[1my8]] - EcBlac + boronic acid inhibitor<br />
+
-
[[1pi4]], [[1pi5]], [[2ffy]] - EcBlac (mutant) + boronic acid inhibitor<br />
+
-
[[1l2s]], [[1xgi]], [[1xgj]], [[2hdq]], [[2hdr]], [[2hds]], [[2hdu]], [[2p9v]], [[2pu2]], [[2pu4]], [[2r9w]], [[2r9x]], [[2rcx]] - EcBlac + inhibitor<br />
+
-
[[1o07]] - EcBlac (mutant) + inhibitor<br />
+
-
[[3s1y]], [[3s22]] - PaBlac (mutant) + inhibitor<br />
+
-
 
+
-
===Non-classified β-lactamase===
+
-
 
+
-
[[1bsg]] – Blac – ''Streptomyces albus''<br />
+
-
[[1alq]], [[1blp]], [[1dja]], [[1djb]], [[1djc]], [[1ghi]] – SaBlac (mutant) <br />
+
-
[[1gce]], [[1xx2]] - EncBlac <br />
+
-
[[1dy6]] – SmBlac Sme-1<br />
+
-
[[1fr1]], [[1fr6]] – CfBlac <br />
+
-
[[1i2s]] – BlBlac<br />
+
-
[[2gdn]] – MtBlac – ''Mycobacterium tuberculosis''<br />
+
-
[[2jc7]], [[3g4p]] – AbBlac – ''Acinetobacter baumannii''<br />
+
-
[[3byd]] – Blac – ''Klebsiella oxytoca''<br />
+
-
[[3i7j]] – Blac – ''Mycobacterium bovis''<br />
+
-
[[3lez]] – Blac – ''Oceanobacter iheyensis''<br />
+
-
[[3p09]] – Blac – ''Francisella tularensis''<br />
+
-
[[4blm]] - BlBlac
+
-
 
+
-
===Non-classified β-lactamase binary complex===
+
-
 
+
-
[[1ghm]], [[1ghp]], [[1pio]] - SaBlac (mutant) + antibiotic<br />
+
-
[[1blc]], [[1blh]]– SaBlac + inhibitor<br />
+
-
[[1bls ]]– EncBlac + phosphonate derivative <br />
+
-
[[1bul]] - EncBlac + penicillin derivative<br />
+
-
[[1ga0]] - EncBlac + inhibitor<br />
+
-
[[1i2w]] – BlBlac + cifoxitin<br />
+
-
[[3cg5]] – MtBlac + antibiotic<br />
+
-
[[3n8s]], [[3ny4]], [[3n6i]], [[3n7w]], [[3n8l]], [[3n8r]], [[3nbl]], [[3nc8]], [[3nck]], [[3nde]], [[3ndg]] - MtBlac (mutant) + antibiotic<br />
+
-
[[3dwz]], [[3iqa]], [[3m6b]], [[3m6h]] – MtBlac + inhibitor<br />
+
-
[[3fv7]], [[3fyz]], [[3fzc]], [[3mbz]] – AbBlac + inhibitor<br />
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
 +
== References ==
 +
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Class B β-lactamase Ndm-1 complex with Zn+2 (grey) (PDB code 3spu)

Drag the structure with the mouse to rotate

References

  1. ↑ Jacoby GA. AmpC beta-lactamases. Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents. PMID:19136439 doi:10.1128/CMR.00036-08
  2. ↑ King D, Strynadka N. Crystal structure of New Delhi metallo-beta-lactamase reveals molecular basis for antibiotic resistance. Protein Sci. 2011 Sep;20(9):1484-91. doi: 10.1002/pro.697. Epub 2011 Aug, 2. PMID:21774017 doi:10.1002/pro.697

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Jaime Prilusky, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/Beta-lactamase"
Personal tools