Enoylpyruvate transferase

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<StructureSection load='3kr6' size='350' side='right' caption='Structure of enoylpyruvate transferase complex with PEP, UDP-N-acetylglucosamine, glycerol and sulfate (PDB entry [[3swe]])' scene=''>
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<StructureSection load='3kr6' size='350' side='right' caption='Structure of enoylpyruvate transferase complex with fosfomycin and UDP-N-acetylglucosamine (PDB entry [[3kr6]])' scene='55/551189/Cv/1'>
== Function ==
== Function ==
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'''Enoylpyruvate transferase''' (MurA) catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls. MurA is composed of catalytic domain and C-terminal domain<ref>PMID:7608103</ref>.
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'''Enoylpyruvate transferase''' ('''MurA''') or '''UDP-N-acetylglucosamine 1-carboxyvinyltransferase''' catalyzes the ligation of phosphoenolpyruvate (PEP) to UDP-N-acetylglucosamine (UNAG). The pyruvate moiety makes the linker between the glycan and peptide portion of peptidoglycans. Thus MurA is essential for the biosynthesis of bacterial cell walls.<ref>PMID:7608103</ref>.
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*'''MurC''' or '''UDP-N-acetylmuramate-L-alanine ligase''' adds L-alanine in the biosynthesis of peptidoglycans which form part of the protective bacterial cell wall<ref>PMID:25114134</ref>.
== Relevance ==
== Relevance ==
MurA is a target for antibiotics such as fosfomycin.
MurA is a target for antibiotics such as fosfomycin.
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</StructureSection>
 
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== 3D Structures of enoylpyruvate transferase ==
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== Structural highlights ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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MurA is composed of <scene name='55/551189/Cv/9'>catalytic domain</scene> and <scene name='55/551189/Cv/10'>C-terminal domain</scene>. The active site is located at the interface of the two domains and binds the <scene name='55/551189/Cv/15'>fosfomycin</scene> and <scene name='55/551189/Cv/20'>UDP-GlcNAc</scene>.<ref>PMID:8994972</ref> Water molecules are shown as red spheres.
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*MurA
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== 3D Structures of enoylpyruvate transferase ==
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[[Enoylpyruvate transferase 3D structures]]
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**[[1naw]], [[1ejc]], [[1ejd]] – EncMurA – ''Enterobacter cloacae''<BR />
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</StructureSection>
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**[[1dlg]], [[3spb]], [[3v5v]], [[4e7g]], [[4eii]] – EncMurA (mutant) <BR />
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**[[1uae]] – EcMurA – ''Escherichia coli''<BR />
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**[[2yvw]] – AaMurA – ''Aquifex aeolicus''<BR />
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**[[3zh3]], [[3zh4]] – MurA – ''Streptococcus pneumoniae''<BR />
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**[[3r38]] – MurA – ''Listeria monocytogenes''<br />
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**[[3sg1]] – MurA – ''Bacillus anthracis''<br />
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*MurA binary complex
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**[[1a2n]] – EcMurA (mutant) + reaction intermediate<BR />
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**[[3iss]] – EcMurA (mutant) + enoylpyruvyl-UNAG <BR />
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**[[1eyn]] – EncMurA (mutant) + aniline-naphthalene sulfonate<BR />
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**[[4e7b]] – EncMurA + UDP-glucose<BR />
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**[[4e7e]] – EncMurA (mutant) + UDP-glucose<BR />
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**[[4e7c]] – EncMurA + UTP<BR />
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**[[4e7d]] – EncMurA + UDP<BR />
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**[[4e7f]] – EncMurA (mutant) + UDP<BR />
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**[[1q3g]] – EncMurA (mutant) + reaction intermediate<BR />
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**[[2rl1]] – HiMurA + UNAG – Haemophilus influenzae<BR />
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**[[3kqj]] – EcMurA + UNAG <BR />
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**[[3upk]] – EncMurA + UNAG <BR />
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**[[1ybg]] – EcMurA (mutant) + inhibitor<BR />
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**[[3kqa]] – EncMurA + terreic acid inhibitor<BR />
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**[[3swq]] – EncMurA (mutant) + enoylpyruvyl-UNAG <BR />
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**[[3v4t]], [[1ryw]] – EncMurA (mutant) + UNAG <BR />
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*MurA ternary complex with antibiotics
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**[[3kr6]] – EcMurA + UNAG + fosfomycin<BR />
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**[[3lth]] – EncMurA + UNAG + fosfomycin<BR />
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**[[3vcy]] – MurA + UNAG + fosfomycin – ''Vibrio fischeri''<BR />
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**[[2rl2]] – HiMurA + UNAG + fosfomycin<BR />
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**[[2z2c]] – EcMurA + UNAG + cnicin<BR />
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*MurA ternary complex with PEP
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**[[3su9]], [[3swa]], [[3swi]] – EncMurA (mutant) + UNAG + PEP<BR />
 
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**[[3swd]] – EcMurA (mutant) + UNAG + PEP<BR />
 
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**[[3swe]] – HiMurA (mutant) + UNAG + PEP<BR />
 
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**[[3swg]] – AaMurA + UNAG + PEP<BR />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of enoylpyruvate transferase complex with fosfomycin and UDP-N-acetylglucosamine (PDB entry 3kr6)

Drag the structure with the mouse to rotate

References

  1. ↑ Brown ED, Vivas EI, Walsh CT, Kolter R. MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli. J Bacteriol. 1995 Jul;177(14):4194-7. PMID:7608103
  2. ↑ Humnabadkar V, Prabhakar KR, Narayan A, Sharma S, Guptha S, Manjrekar P, Chinnapattu M, Ramachandran V, Hameed SP, Ravishankar S, Chatterji M. UDP-N-acetylmuramic acid l-alanine ligase (MurC) inhibition in a tolC mutant Escherichia coli strain leads to cell death. Antimicrob Agents Chemother. 2014 Oct;58(10):6165-71. doi: 10.1128/AAC.02890-14. , Epub 2014 Aug 11. PMID:25114134 doi:http://dx.doi.org/10.1128/AAC.02890-14
  3. ↑ Skarzynski T, Mistry A, Wonacott A, Hutchinson SE, Kelly VA, Duncan K. Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin. Structure. 1996 Dec 15;4(12):1465-74. PMID:8994972

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