1hbw

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[[Image:1hbw.jpg|left|200px]]
 
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==Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)==
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The line below this paragraph, containing "STRUCTURE_1hbw", creates the "Structure Box" on the page.
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<StructureSection load='1hbw' size='340' side='right'caption='[[1hbw]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HBW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hbw OCA], [https://pdbe.org/1hbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hbw RCSB], [https://www.ebi.ac.uk/pdbsum/1hbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hbw ProSAT]</span></td></tr>
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{{STRUCTURE_1hbw| PDB=1hbw | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAL4_YEAST GAL4_YEAST] This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GAL4 dimerization domain (GAL4-dd) is a powerful transcriptional activator when tethered to DNA in a cell bearing a mutant of the GAL11 protein, named GAL11P. GAL11P (like GAL11) is a component of the RNA-polymerase II holoenzyme. Nuclear magnetic resonance (NMR) studies of GAL4-dd revealed an elongated dimer structure with C(2) symmetry containing three helices that mediate dimerization via coiled-coil contacts. The two loops between the three coiled coils form mobile bulges causing a variation of twist angles between the helix pairs. Chemical shift perturbation analysis mapped the GAL11P-binding site to the C-terminal helix alpha3 and the loop between alpha1 and alpha2. One GAL11P monomer binds to one GAL4-dd dimer rendering the dimer asymmetric and implying an extreme negative cooperativity mechanism. Alanine-scanning mutagenesis of GAL4-dd showed that the NMR-derived GAL11P-binding face is crucial for the novel transcriptional activating function of the GAL4-dd on GAL11P interaction. The binding of GAL4 to GAL11P, although an artificial interaction, represents a unique structural motif for an activating region capable of binding to a single target to effect gene expression.
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'''SOLUTION NMR STRUCTURE OF THE DIMERIZATION DOMAIN OF THE YEAST TRANSCRIPTIONAL ACTIVATOR GAL4 (RESIDUES 50-106)'''
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Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain.,Hidalgo P, Ansari AZ, Schmidt P, Hare B, Simkovich N, Farrell S, Shin EJ, Ptashne M, Wagner G Genes Dev. 2001 Apr 15;15(8):1007-20. PMID:11316794<ref>PMID:11316794</ref>
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==Overview==
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The GAL4 dimerization domain (GAL4-dd) is a powerful transcriptional activator when tethered to DNA in a cell bearing a mutant of the GAL11 protein, named GAL11P. GAL11P (like GAL11) is a component of the RNA-polymerase II holoenzyme. Nuclear magnetic resonance (NMR) studies of GAL4-dd revealed an elongated dimer structure with C(2) symmetry containing three helices that mediate dimerization via coiled-coil contacts. The two loops between the three coiled coils form mobile bulges causing a variation of twist angles between the helix pairs. Chemical shift perturbation analysis mapped the GAL11P-binding site to the C-terminal helix alpha3 and the loop between alpha1 and alpha2. One GAL11P monomer binds to one GAL4-dd dimer rendering the dimer asymmetric and implying an extreme negative cooperativity mechanism. Alanine-scanning mutagenesis of GAL4-dd showed that the NMR-derived GAL11P-binding face is crucial for the novel transcriptional activating function of the GAL4-dd on GAL11P interaction. The binding of GAL4 to GAL11P, although an artificial interaction, represents a unique structural motif for an activating region capable of binding to a single target to effect gene expression.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1HBW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBW OCA].
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</div>
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<div class="pdbe-citations 1hbw" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain., Hidalgo P, Ansari AZ, Schmidt P, Hare B, Simkovich N, Farrell S, Shin EJ, Ptashne M, Wagner G, Genes Dev. 2001 Apr 15;15(8):1007-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11316794 11316794]
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*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Ansari AZ]]
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[[Category: Ansari, A Z.]]
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[[Category: Farrell S]]
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[[Category: Farrell, S.]]
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[[Category: Hare B]]
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[[Category: Hare, B.]]
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[[Category: Hidalgo P]]
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[[Category: Hidalgo, P.]]
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[[Category: Ptashne M]]
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[[Category: Ptashne, M.]]
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[[Category: Schmidt P]]
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[[Category: Schmidt, P.]]
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[[Category: Shin EJ]]
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[[Category: Shin, E J.]]
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[[Category: Simkovic N]]
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[[Category: Simkovic, N.]]
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[[Category: Wagner G]]
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[[Category: Wagner, G.]]
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[[Category: Coiled-coil homodimer]]
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[[Category: Dimerization domain]]
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[[Category: Galactose and melibiose metabolism]]
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[[Category: Transcriptional activator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:40:44 2008''
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Current revision

Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)

PDB ID 1hbw

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