1hbw

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(New page: 200px<br /><applet load="1hbw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hbw" /> '''SOLUTION NMR STRUCTURE OF THE DIMERIZATION D...)
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[[Image:1hbw.jpg|left|200px]]<br /><applet load="1hbw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hbw" />
 
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'''SOLUTION NMR STRUCTURE OF THE DIMERIZATION DOMAIN OF THE YEAST TRANSCRIPTIONAL ACTIVATOR GAL4 (RESIDUES 50-106)'''<br />
 
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==Overview==
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==Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)==
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The GAL4 dimerization domain (GAL4-dd) is a powerful transcriptional, activator when tethered to DNA in a cell bearing a mutant of the GAL11, protein, named GAL11P. GAL11P (like GAL11) is a component of the, RNA-polymerase II holoenzyme. Nuclear magnetic resonance (NMR) studies of, GAL4-dd revealed an elongated dimer structure with C(2) symmetry, containing three helices that mediate dimerization via coiled-coil, contacts. The two loops between the three coiled coils form mobile bulges, causing a variation of twist angles between the helix pairs. Chemical, shift perturbation analysis mapped the GAL11P-binding site to the, C-terminal helix alpha3 and the loop between alpha1 and alpha2. One GAL11P, monomer binds to one GAL4-dd dimer rendering the dimer asymmetric and, implying an extreme negative cooperativity mechanism. Alanine-scanning, mutagenesis of GAL4-dd showed that the NMR-derived GAL11P-binding face is, crucial for the novel transcriptional activating function of the GAL4-dd, on GAL11P interaction. The binding of GAL4 to GAL11P, although an, artificial interaction, represents a unique structural motif for an, activating region capable of binding to a single target to effect gene, expression.
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<StructureSection load='1hbw' size='340' side='right'caption='[[1hbw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HBW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hbw OCA], [https://pdbe.org/1hbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hbw RCSB], [https://www.ebi.ac.uk/pdbsum/1hbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hbw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAL4_YEAST GAL4_YEAST] This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GAL4 dimerization domain (GAL4-dd) is a powerful transcriptional activator when tethered to DNA in a cell bearing a mutant of the GAL11 protein, named GAL11P. GAL11P (like GAL11) is a component of the RNA-polymerase II holoenzyme. Nuclear magnetic resonance (NMR) studies of GAL4-dd revealed an elongated dimer structure with C(2) symmetry containing three helices that mediate dimerization via coiled-coil contacts. The two loops between the three coiled coils form mobile bulges causing a variation of twist angles between the helix pairs. Chemical shift perturbation analysis mapped the GAL11P-binding site to the C-terminal helix alpha3 and the loop between alpha1 and alpha2. One GAL11P monomer binds to one GAL4-dd dimer rendering the dimer asymmetric and implying an extreme negative cooperativity mechanism. Alanine-scanning mutagenesis of GAL4-dd showed that the NMR-derived GAL11P-binding face is crucial for the novel transcriptional activating function of the GAL4-dd on GAL11P interaction. The binding of GAL4 to GAL11P, although an artificial interaction, represents a unique structural motif for an activating region capable of binding to a single target to effect gene expression.
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==About this Structure==
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Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain.,Hidalgo P, Ansari AZ, Schmidt P, Hare B, Simkovich N, Farrell S, Shin EJ, Ptashne M, Wagner G Genes Dev. 2001 Apr 15;15(8):1007-20. PMID:11316794<ref>PMID:11316794</ref>
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1HBW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HBW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain., Hidalgo P, Ansari AZ, Schmidt P, Hare B, Simkovich N, Farrell S, Shin EJ, Ptashne M, Wagner G, Genes Dev. 2001 Apr 15;15(8):1007-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11316794 11316794]
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</div>
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[[Category: Saccharomyces cerevisiae]]
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<div class="pdbe-citations 1hbw" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Ansari, A.Z.]]
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[[Category: Farrell, S.]]
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[[Category: Hare, B.]]
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[[Category: Hidalgo, P.]]
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[[Category: Ptashne, M.]]
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[[Category: Schmidt, P.]]
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[[Category: Shin, E.J.]]
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[[Category: Simkovic, N.]]
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[[Category: Wagner, G.]]
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[[Category: coiled-coil homodimer]]
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[[Category: dimerization domain]]
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[[Category: galactose and melibiose metabolism]]
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[[Category: transcriptional activator]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:30:14 2007''
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==See Also==
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*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Ansari AZ]]
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[[Category: Farrell S]]
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[[Category: Hare B]]
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[[Category: Hidalgo P]]
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[[Category: Ptashne M]]
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[[Category: Schmidt P]]
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[[Category: Shin EJ]]
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[[Category: Simkovic N]]
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[[Category: Wagner G]]

Current revision

Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)

PDB ID 1hbw

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