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| | ==Identification and structural characterization of LytU== | | ==Identification and structural characterization of LytU== |
| - | <StructureSection load='5kqc' size='340' side='right' caption='[[5kqc]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='5kqc' size='340' side='right'caption='[[5kqc]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kqc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KQC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KQC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kqc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KQC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lytM_3, AS858_11005, ERS093009_01996 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysostaphin Lysostaphin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.75 3.4.24.75] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kqc OCA], [https://pdbe.org/5kqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kqc RCSB], [https://www.ebi.ac.uk/pdbsum/5kqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kqc ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kqc OCA], [http://pdbe.org/5kqc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kqc RCSB], [http://www.ebi.ac.uk/pdbsum/5kqc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kqc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2G1F1_STAA8 Q2G1F1_STAA8] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lysostaphin]] | + | [[Category: Large Structures]] |
| - | [[Category: Permi, P]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Raulinaitis, V]] | + | [[Category: Permi P]] |
| - | [[Category: Tossavainen, H]] | + | [[Category: Raulinaitis V]] |
| - | [[Category: Hydrolase]] | + | [[Category: Tossavainen H]] |
| - | [[Category: Lytu]]
| + | |
| - | [[Category: Peptidoglycan]]
| + | |
| - | [[Category: Zinc]]
| + | |
| Structural highlights
Function
Q2G1F1_STAA8
Publication Abstract from PubMed
We introduce LytU, a short member of the lysostaphin family of zinc-dependent pentaglycine endopeptidases. It is a potential antimicrobial agent for S. aureus infections and its gene transcription is highly upregulated upon antibiotic treatments along with other genes involved in cell wall synthesis. We found this enzyme to be responsible for the opening of the cell wall peptidoglycan layer during cell divisions in S. aureus. LytU is anchored in the plasma membrane with the active part residing in the periplasmic space. It has a unique Ile/Lys insertion at position 151 that resides in the catalytic site-neighbouring loop and is vital for the enzymatic activity but not affecting the overall structure common to the lysostaphin family. Purified LytU lyses S. aureus cells and cleaves pentaglycine, a reaction conveniently monitored by NMR spectroscopy. Substituting the cofactor zinc ion with a copper or cobalt ion remarkably increases the rate of pentaglycine cleavage. NMR and isothermal titration calorimetry further reveal that, uniquely for its family, LytU is able to bind a second zinc ion which is coordinated by catalytic histidines and is therefore inhibitory. The pH-dependence and high affinity of binding carry further physiological implications.
Identification and structural characterization of LytU, a unique peptidoglycan endopeptidase from the lysostaphin family.,Raulinaitis V, Tossavainen H, Aitio O, Juuti JT, Hiramatsu K, Kontinen V, Permi P Sci Rep. 2017 Jul 20;7(1):6020. doi: 10.1038/s41598-017-06135-w. PMID:28729697[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Raulinaitis V, Tossavainen H, Aitio O, Juuti JT, Hiramatsu K, Kontinen V, Permi P. Identification and structural characterization of LytU, a unique peptidoglycan endopeptidase from the lysostaphin family. Sci Rep. 2017 Jul 20;7(1):6020. doi: 10.1038/s41598-017-06135-w. PMID:28729697 doi:http://dx.doi.org/10.1038/s41598-017-06135-w
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