HypA-HypB-HypC-HypD-HypE-HypF
From Proteopedia
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<StructureSection load='2z1d' size='340' side='right' caption='HypD with Fe4S4 center (PDB code [[2z1d]])' scene=''> | <StructureSection load='2z1d' size='340' side='right' caption='HypD with Fe4S4 center (PDB code [[2z1d]])' scene=''> | ||
== Function == | == Function == | ||
| - | The [NiFe] hydrogenase contains a NiFe(CO)(CN)2 in its reaction center. '''HypA, HypB, HypC, HypD, HypE and HypF''' are required for the maturation of [NiFe] hydrogenase. '''HypA'''<ref>PMID:15995183</ref> and '''HypB'''<ref>PMID:9140970</ref> are involved in the insertion of Ni atom. '''HypE''' and '''HypF'''<ref>PMID:15291820</ref> catalyze the biosynthesis of the CN ligand. '''HypC''' and '''HypD'''<ref>PMID:15504408</ref> deliver the Fe and CO to the hydrogenase active center. | + | The [NiFe] hydrogenase or '''hydrogenase expression/formation protein''' contains a NiFe(CO)(CN)2 in its reaction center. '''HypA, HypB, HypC, HypD, HypE and HypF''' are required for the maturation of [NiFe] hydrogenase. |
| + | *'''HypA'''<ref>PMID:15995183</ref> and '''HypB'''<ref>PMID:9140970</ref> are involved in the insertion of Ni atom. For more details of HypA see [[Molecular Playground/HypA]]. | ||
| + | *'''HypE''' and '''HypF'''<ref>PMID:15291820</ref> catalyze the biosynthesis of the CN ligand. | ||
| + | *'''HypC''' and '''HypD'''<ref>PMID:15504408</ref> deliver the Fe and CO to the hydrogenase active center. | ||
== 3D Structures of HypA, HypB, HypC, HypD, HypE and HypF == | == 3D Structures of HypA, HypB, HypC, HypD, HypE and HypF == | ||
Current revision
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References
- ↑ Atanassova A, Zamble DB. Escherichia coli HypA is a zinc metalloprotein with a weak affinity for nickel. J Bacteriol. 2005 Jul;187(14):4689-97. PMID:15995183 doi:http://dx.doi.org/10.1128/JB.187.14.4689-4697.2005
- ↑ Olson JW, Fu C, Maier RJ. The HypB protein from Bradyrhizobium japonicum can store nickel and is required for the nickel-dependent transcriptional regulation of hydrogenase. Mol Microbiol. 1997 Apr;24(1):119-28. PMID:9140970
- ↑ Blokesch M, Paschos A, Bauer A, Reissmann S, Drapal N, Bock A. Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE. Eur J Biochem. 2004 Aug;271(16):3428-36. PMID:15291820 doi:http://dx.doi.org/10.1111/j.1432-1033.2004.04280.x
- ↑ Blokesch M, Albracht SP, Matzanke BF, Drapal NM, Jacobi A, Bock A. The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases. J Mol Biol. 2004 Nov 12;344(1):155-67. PMID:15504408 doi:http://dx.doi.org/10.1016/j.jmb.2004.09.040
