8kcl
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - Triclinic form== | |
| + | <StructureSection load='8kcl' size='340' side='right'caption='[[8kcl]], [[Resolution|resolution]] 1.47Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8kcl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ligilactobacillus_ruminis_ATCC_25644 Ligilactobacillus ruminis ATCC 25644]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8KCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8KCL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.47Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8kcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8kcl OCA], [https://pdbe.org/8kcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8kcl RCSB], [https://www.ebi.ac.uk/pdbsum/8kcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8kcl ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E7FRT5_9LACO E7FRT5_9LACO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sortase-dependent pili are long surface appendages that mediate attachment, colonization and biofilm formation in certain genera and species of Gram-positive bacteria. Ligilactobacillus ruminis is an autochthonous gut commensal that relies on sortase-dependent LrpCBA pili for host adherence and persistence. X-ray crystal structure snapshots of the backbone pilin LrpA were captured in two atypical bent conformations leading to a zigzag morphology in the LrpCBA pilus structure. Small-angle X-ray scattering and structural analysis revealed that LrpA also adopts the typical linear conformation, resulting in an elongated pilus morphology. Various conformational analyses and biophysical experiments helped to demonstrate that a hinge region located at the end of the flexible N-terminal domain of LrpA facilitates a new closure-and-twist motion for assembling dynamic pili during the assembly process and host attachment. Further, the incongruent combination of flexible domain-driven conformational dynamics and rigid isopeptide bond-driven stability observed in the LrpCBA pilus might also extend to the sortase-dependent pili of other bacteria colonizing a host. | ||
| - | + | The crystal structure of the N-terminal domain of the backbone pilin LrpA reveals a new closure-and-twist motion for assembling dynamic pili in Ligilactobacillus ruminis.,Prajapati A, Palva A, von Ossowski I, Krishnan V Acta Crystallogr D Struct Biol. 2024 Jul 1;80(Pt 7):474-492. doi: , 10.1107/S2059798324005114. Epub 2024 Jun 27. PMID:38935340<ref>PMID:38935340</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8kcl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ligilactobacillus ruminis ATCC 25644]] | ||
| + | [[Category: Krishnan V]] | ||
| + | [[Category: Palva A]] | ||
| + | [[Category: Prajapati A]] | ||
| + | [[Category: Von Ossowski I]] | ||
Current revision
Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - Triclinic form
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