RNA uridylyltransferase

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[[Image:3pq1.png|left|200px|thumb|Crystal Structure of SeMet human RNA uridylyltransferase-1 [[3pq1]]]]
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<StructureSection load='' size='350' side='right' caption='RNA uridylyltransferase-4 complex with UTP, UMP and Mg+2 ions (green) (PDB entry [[2q0f]])' scene='53/536705/Cv/1'>
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{{STRUCTURE_3pq1| PDB=3pq1 | SIZE=400| SCENE= |right|CAPTION=SeMet Human RNA uridylyltransferase-1, [[3pq1]] }}
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== Function ==
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'''RNA uridylyltransferase''' or '''terminal uridylyltransferase''' or '''TUTase''' (TUT) catalyzes the addition of uridine to RNA. TUT acts as a suppressor of microRNA synthesis by mediating its terminal uridylation<ref>PMID:17189640</ref>. For additional details on TUT-4 see [[Terminal Uridylyl Transferase]].
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* ''Drosophila melanogaster'' TUTase is called '''TUTase Tailor'''. It is forming the uridylation-mediated processing complex which degrades a variety ofncRNAs in the cytoplasm <ref>PMID:26145174</ref>,.<ref>PMID:27729457</ref>
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== Structural highlights ==
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<scene name='53/536705/Cv/5'>TUT-4 active site contains UTP, UMP and Mg+2 ions</scene><ref>PMID:17785418</ref>. Water molecules are shown as red spheres. <scene name='53/536705/Cv/6'>Mg coordination site</scene>.
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</StructureSection>
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== 3D Structures of RNA uridylyltransferase ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*RNA uridylyltransferase-1
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**[[2e5g]] – hTUT-1 RNA recognition motif – human – NMR<br />
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**[[3pq1]] – hTUT-1 (mutant)<br />
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**[[6l3f]] – AtTUT-1 (mutant) – ''Arabidopsis thaliana''<br />
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**[[6l8k]] – AtTUT-1 + UTP<br />
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**[[2b51]], [[5hcd]] – TbTUT-1 – ''Trypanosoma brucei''<br />
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**[[5ido]] – TbTUT-1 + UTP <br />
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**[[5i49]] – TbTUT-1 + UMPNPP <br />
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*RNA uridylyltransferase-4
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**[[6iw6]] – hTUT-4 253-723<br />
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**[[2q0c]], [[2q0d]], [[2q0e]], [[2q0f]], [[2q0g]], [[2ikf]], [[2nom]], [[5kal]] – TbTUT-4 + nucleotide <br />
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*RNA uridylyltransferase-7
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**[[5w0b]] – hTUT-7 catalytic module<br />
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**[[5w0m]] – hTUT-7 + U5 ssRNA <br />
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**[[5w0o]] – hTUT-7 + dsRNA + UTPbr />
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**[[5w0n]] – hTUT-7 + U2 ssRNA + UMPNPP<br />
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*TUTase Tailor
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**[[5z4c]] – DmTUTT – ''Drosophila melanogaster'' <br />
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**[[5z4a]], [[5z4d]], [[5z4j]], [[6i0t]], [[6i0u]], [[6i0v]] – DmTUTT + RNA <br />
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**[[5z4m]] – DmTUTT (mutant) + UTP <br />
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}}
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== References ==
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<references/>
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[[Category:Topic Page]]
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'''RNA uridylyltransferase''' (RNAU) catalyzes the addition of uridine to RNA. RNAU acts as a suppressor of microRNA synthesis by mediating its terminal uridylation. For additional details on RNAU-4 see [[Terminal Uridylyl Transferase]].
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== 3D Structures of RNA uridylyltransferase ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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===RNA uridylyltransferase-1===
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[[2e5g]] – hRNAU-1 RNA recognition motif – human – NMR<br />
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[[3pq1]] – hRNAU-1 (mutant)<br />
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===RNA uridylyltransferase-4===
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[[2q0c]], [[2q0d]], [[2q0e]], [[2q0f]], [[2q0g]], [[2ikf]], [[2nom]] – TbRNAU-4 + nucleotide – ''Trypanosoma brucei''<br />
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Current revision

RNA uridylyltransferase-4 complex with UTP, UMP and Mg+2 ions (green) (PDB entry 2q0f)

Drag the structure with the mouse to rotate

3D Structures of RNA uridylyltransferase

Updated on 11-August-2024

References

  1. ↑ Stagno J, Aphasizheva I, Rosengarth A, Luecke H, Aphasizhev R. UTP-bound and Apo structures of a minimal RNA uridylyltransferase. J Mol Biol. 2007 Feb 23;366(3):882-99. Epub 2006 Dec 2. PMID:17189640 doi:10.1016/j.jmb.2006.11.065
  2. ↑ Bortolamiol-Becet D, Hu F, Jee D, Wen J, Okamura K, Lin CJ, Ameres SL, Lai EC. Selective Suppression of the Splicing-Mediated MicroRNA Pathway by the Terminal Uridyltransferase Tailor. Mol Cell. 2015 Jul 16;59(2):217-28. doi: 10.1016/j.molcel.2015.05.034. Epub 2015 , Jul 2. PMID:26145174 doi:http://dx.doi.org/10.1016/j.molcel.2015.05.034
  3. ↑ Reimão-Pinto MM, Manzenreither RA, Burkard TR, Sledz P, Jinek M, Mechtler K, Ameres SL. Molecular basis for cytoplasmic RNA surveillance by uridylation-triggered decay in Drosophila. EMBO J. 2016 Nov 15;35(22):2417-2434. PMID:27729457 doi:10.15252/embj.201695164
  4. ↑ Stagno J, Aphasizheva I, Aphasizhev R, Luecke H. Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases. Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14634-9. Epub 2007 Sep 4. PMID:17785418

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